The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

文献类型： 外文期刊

第一作者： Bai Li-jing

作者： Bai Li-jing;Ju Hui-ming;Mu Yu-Lian;Yang Shu-lin;Ren Hong-yan;Ao Hong;Li Kui;Bai Li-jing;Wang Chu-duan;Ju Hui-ming

作者机构：

关键词： type A spermatogonial stem cells;dicistronic expression vector;co-expression;transgenic mice

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pcDNA-appA-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testis-mediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89% (16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by the appA gene (P<0.05); these animals also exhibited a strong anti-viral phenotype.

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