Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs

文献类型： 外文期刊

第一作者： Zhao Shuan-ping

作者： Zhao Shuan-ping;Tang Zhong-lin;Zhou Rong;Li Kui;Zheng Jian-wei;Qu Chang-qing;Zhao Shuan-ping

作者机构：

关键词： pig;CRABP1;chromosome assignment;expression profile;allele frequency

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Cellular retinoic acid-binding protein 1 (CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression (LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7000 porcine radiation hybrid panel (IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle (33, 65 and 90 days post coitus, dpc) from Landrace (lean-type) (described as L33, L65 and L90) and Tongcheng pigs (obese-type) (described as T33, T65 and T90). The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms (SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 (G>A) and g. 2992 (G>A) were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY (Durocx(LandracexYorkshire)) pig breeds.

分类号：

  • 相关文献

[1]Molecular Characterization of Tob1 in Muscle Development in Pigs. Yuan, Jing,Cao, Ji-Yue,Yuan, Jing,Tang, Zhong-Lin,Li, Kui,Wang, Ning. 2011

[2]A substitution within erythropoietin receptor gene D1 domain associated with litter size in Beijing Black pig, Sus scrofa. Zhang, Longchao,Wang, Ligang,Li, Yong,Li, Wen,Yan, Hua,Liu, Xin,Zhao, Kebin,Wang, Lixian.

[3]Allelic diversity and geographical pattern at soybean Kunitz trypsin inhibitor single locus in Chinese wild soybean (Glycine soja Sieb. & Zucc.). Wang, K. J.,Li, X. H.,Takahata, Y.,Yamashita, T..

[4]Frequency detection of imidacloprid resistance allele in Aphis gossypii field populations by real-time PCR amplification of specific-allele (rtPASA). Cui, Li,Xu, Xibao,Rui, Changhui.

[5]Isolation and Characterization of 54 Polymorphic Microsatellite Markers in Scylla paramamosain by FIASCO Approach. Ma, Hongyu,Ma, Chunyan,Ma, Lingbo,Zhang, Fengying,Qiao, Zhenguo.

[6]Status of insecticide resistance and associated mutations in Q-biotype of whitefly, Bemisia tabaci, from eastern China. Yuan, Linze,Du, Yuzhou,Wang, Jianjun,Wang, Shaoli,Zhang, Youjun,Zhou, Jiachun. 2012

[7]Polymorphism of polygalacturonase-inhibiting protein genes by fluorescence-based PCR single-strand conformation profiling and sequencing in autotetraploid alfalfa (Medicago sativa) populations. Gao, Jianming,Wang, Yu,Yuan, Qinghua,Li, Xianglin,Gao, Jianming,Gui, Zhi,Liu, Huiqin,Xin, Nan,Zhang, Xin,Chen, Xiaoqiang.

[8]The PpLTP1 Primary Allergen Gene is Highly Conserved in Peach and Has Small Variations in Other Prunus Species. Zhou, Xiang,Gao, Zhong-shan,Li, Xiong-wei,Jia, Hui-juan,Wu, Hong-xia,Xie, Rang-jin,Gao, Zhong-shan,Wang, Zhi-qiang,Cao, Ke,Yu, Ming-liang,Chen, Shuang-jian,Li, Ying-hui,Wang, Hui-ying,van Ree, Ronald.

[9]Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations. Xu, Z. L.,Ali, Z.,Yi, J. X.,He, X. L.,Zhang, D. Y.,Yu, G. H.,Ma, H. X.,Ali, Z.,Khan, A. A.,Ali, Z.,Khan, A. A.,Khan, I. A.. 2011

[10]Association of CAST Gene Polymorphisms with Carcass and Meat Quality Traits in Chinese Commercial Cattle Herds. Xu, Shang-Zhong.

[11]Frequency of Bt Resistance Alleles in Helicoverpa armigera in the Xinjiang Cotton-Planting Region of China. Gao, Yulin,Wu, Kongming,Li, Guoping,Feng, Hongqiang,Wyckhuys, Kris A. C..

[12]A core collection and mini core collection of Oryza sativa L. in China. Zhang, Hongliang,Zhang, Dongling,Wang, Meixing,Sun, Junli,Qi, Yongwen,Li, Jinjie,Li, Zichao,Wei, Xinghua,Tang, Shengxiang,Han, Longzhi,Qiu, Zongen.

[13]Genome-wide analysis of the WRKY gene family in cotton. Dou, Lingling,Zhang, Xiaohong,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Yu, Shuxun.

[14]Sequence Analysis and Molecular Characterization of Wnt4 Gene in Metacestodes of Taenia solium. Hou, Junling,Luo, Xuenong,Wang, Shuai,Yin, Cai,Zhang, Shaohua,Zhu, Xueliang,Dou, Yongxi,Cai, Xuepeng.

[15]Cloning, Characterization, and Expression Analysis of a Gene Encoding a Putative Lysophosphatidic Acid Acyltransferase from Seeds of Paeonia rockii. Zhang, Qing-Yu,Niu, Li-Xin,Yu, Rui,Zhang, Xiao-Xiao,Bai, Zhang-Zhen,Zhang, Yan-Long,Duan, Ke,Gao, Qing-Hua.

[16]Identification and expression profile analysis of putative odorant-binding proteins in Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae). Gong, Zhong-Jun,Miao, Jin,Duan, Yun,Jiang, Yue-Li,Li, Tong,Wu, Yu-Qing.

[17]MicroRNA expression profile in different tissues of BALB/c mice in the early phase of Schistosoma japonicum infection. Han, Hongxiao,Peng, Jinbiao,Hong, Yang,Zhang, Min,Han, Yanhui,Fu, Zhiqiang,Shi, Yaojun,Lin, Jiaojiao,Han, Hongxiao,Liu, Dandan,Xu, Jinjun,Tao, Jianping,Peng, Jinbiao.

[18]In vivo expression patterns of microRNAs of Gallid herpesvirus 2 (GaHV-2) during the virus life cycle and development of Marek's disease lymphomas. Zhao, Pu,Dang, Lu,Zhao, Pu,Li, Xiu-Jie,Teng, Man,Dang, Lu,Yu, Zu-Hua,Chi, Jia-Qi,Luo, Jun,Zhao, Pu,Su, Jing-Wei,Zhang, Gai-Ping,Zhang, Gai-Ping.

[19]Molecular characterization of 60 isolated wheat MYB genes and analysis of their expression during abiotic stress. Zhang, Lichao,Zhao, Guangyao,Jia, Jizeng,Liu, Xu,Kong, Xiuying.

[20]FEEDING ON A BEGOMOVIRUS-INFECTED PLANT ENHANCES FECUNDITY VIA INCREASED EXPRESSION OF AN INSULIN-LIKE PEPTIDE IN THE WHITEFLY, MEAM1. Cheng, Lu,Ye, Gong-Yin,Fang, Qi,Guo, Jian-Yang.

作者其他论文 更多>>

微信小程序