In Vitro Conservation and Cryopreservation in National Genebank of China

文献类型： 外文期刊

第一作者： Zhang, Jinmei

作者： Zhang, Jinmei;Xin, Xia;Yin, Guangkun;Lu, Xinxiong;Chen, Xiaoling

作者机构：

关键词： crop germplasm resources;ex situ conservation;tissue culture;pollen;dormant bud;shoot tip;vegetatively propagated crops

期刊名称：II INTERNATIONAL SYMPOSIUM ON PLANT CRYOPRESERVATION

ISSN： 0567-7572

年卷期： 2014 年 1039 卷

页码：

收录情况： SCI

摘要： Crop germplasm resources are very important for sustainable agriculture. Presently, China has set up 43 national field repositories for vegetatively propagated crops, one long-term seed genebank, one genebank for duplicate germplasm, 10 mid-term seed genebanks and two in vitro genebanks. The National Genebank of China was built in 1986, with partial financial support from Rockefeller Foundation and the erstwhile International Board for Plant Genetic Resources. The missions of the National Genebank of China are to conserve the nationwide crop germplasm resources and to share the information with the national breeders and researchers. Present holding of long-term seedbank is 374,627 accessions, belonging to 898 species. The storage temperature is maintained at -18 degrees C with relative humidity lower than 50%. The in vitro conservation and cryopreservation work have been started in the National Genebank of China for vegetatively propagated crop species during last ten years. Until now, we have stored more than 300 accessions in vitro, belonging to 35 crops, such as potato, sweet potato, banana, yam, taro, cassava, ginger, lily, strawberry and so on. Every year, the number of in vitro accessions is enhanced through national collections and exotic introductions. In addition, the cryopreservation is also primarily established by using shoot tips, dormant buds/twigs, and pollen. The cryopreserved shoot tips belong to potato, banana, lily, Petunia, carnation and so on. The cryopreserved dormant buds/twigs belong to mulberry, apple and pear. The pollen cryopreservation works has been attempted on peach, apple and pear. Overall 82 accessions belonging to 13 crops were cryopreserved. A new Genebank would be set up in three to five years, and now we are engaged in developing in vitro conservation and cryopreservation techniques to meet the demands of new Genebank.

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