Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize

文献类型： 外文期刊

第一作者： Zhang Xiao-li

作者： Zhang Xiao-li;Fan Cheng-ming;Li Hong-jie;Wang Xiao-ming;Si Bing-wen;Zhang Xiao-li

作者机构：

关键词： maize;Setosphaeria turcica;Ht2 gene;differential proteomics

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Northern corn leaf blight (NCLB), caused by the heterothallic ascomycete fungus Setosphaeria turcica, is a destructive foliar disease of maize and represents a serious threat to maize production worldwide. A comparative proteomic study was conducted to explore the molecular mechanisms underlying the defense responses of the maize resistant line A619 Ht2 to S. turcica race 13. Leaf proteins were extracted from mock and S. turcica-infected leaves after inoculated for 72 h and analyzed for differentially expressed proteins using two-dimensional electrophoresis and mass spectrometry identification. 137 proteins showed reproducible differences in abundance by more than 2-fold at least, including 50 up-regulated proteins and 87 down-regulated proteins. 48 protein spots were successfully identified by MS analysis, which included 10 unique, 6 up-regulated, 20 down-regulated and 12 disappeared protein spots. These identified proteins were classified into 9 functional groups and involved in multiple functions, particularly in energy metabolism (46%), protein destination and storage (12%), and disease defense (18%). Some defense-related proteins were upregulated such as P-glucosidase, SOD, polyamines oxidase, HSC 70 and PPIases; while the expressions of photosynthesis- and metabolism-related proteins were down-regulated, by inoculation with S. turcica. The results indicated that a complex regulatory network was functioned in interaction between the resistant line A619 Ht2 and S. turcica. The resistance processes of A619 Ht2 mainly resided on directly releasing defense proteins, modulation of primary metabolism, affecting photosyntesis and carbohydrate metabolism.

分类号：

  • 相关文献

[1]Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica, Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation, Invasive Growth, and Mating Behavior. Gu Shou-qin,Yang Yang,Zhang Chang-zhi,Fan Yu,Zhang Xiao-yu,Tian Lan,Hao Zhi-min,Cao Zhi-yan,Gong Xiao-dong,Han Jian-min,Dong Jin-gao,Li Po,Fan Yong-shan. 2013

[2]MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica. Li Po,Gong Xiao-dong,Jia Hui,Fan Yong-shan,Zhang Yun-feng,Han Jian-min,Gu Shou-qin,Dong Jin-gao,Li Po,Fan Yong-shan,Zhang Yun-feng. 2016

[3]Molecular characterization of StpkaC2 and expression patterns of both PKA-c isoforms during the invasive growth of Setosphaeria turcica. Hao, Zhimin,Tong, Yameng,Han, Yue,Wu, Di,Yang, Zheng,Shen, Shen,Gong, Xiaodong,Cao, Zhiyan,Gu, Shouqin,Dong, Jingao,Li, Zhiyong.

[4]StSTE12 is required for the pathogenicity of Setosphaeria turcica by regulating appressorium development and penetration. Gu, Shou-qin,Li, Po,Wu, Min,Hao, Zhi-min,Gong, Xiao-dong,Zhang, Xiao-yu,Tian, Lan,Zhang, Pan,Wang, Yue,Cao, Zhi-yan,Han, Jian-min,Dong, Jin-gao,Li, Po,Fan, Yong-shan.

[5]The catalytic subunit of cAMP-dependent protein kinase A StPKA-c contributes to conidiation and early invasion in the phytopathogenic fungus Setosphaeria turcica. Shen, Shen,Hao, Zhimin,Gu, Shouqin,Wang, Jingjing,Cao, Zhiyan,Dong, Jingao,Li, Zhiyong,Wang, Qian,Li, Po,Hao, Jie.

[6]Proteomic Analysis of the Resistant Responses of Two Vitis amurensis Cultivars to Plasmopara viticola Infections. Xu, Pei-lei,Liu, Ying-xue,Qin, Hong-yan,Ai, Jun,Fan, Shu-tian,Yang, Yi-ming,Zhao, Ying,Li, Xiao-yan,Li, Xiao-hong.

[7]Comparative proteomic analysis reveals alterations in development and photosynthesis-related proteins in diploid and triploid rice. Wang, Shuzhen,Chen, Wenyue,Xiao, Wenfei,Xin, Ya,Qiu, Jieren,Ying, Wu,Tong, Jianxin,Chen, Zhongzhong,Ruan, Songlin,Ma, Huasheng,Wang, Shuzhen,Yang, Changdeng,Fu, Yaping,Wang, Shuzhen,Yao, Jian,Yao, Haigen,Wang, Shuzhen,Hu, Weimin. 2016

[8]Proteomic analysis of leaves of different wheat genotypes subjected to PEG 6000 stress and rewatering. Ye, Jingxiu,Zhang, Fengjun,Xie, Deqing,Yao, Youhua,Wang, Shuping. 2013

[9]Ca2+-binding protein S100A11: A novel diagnostic marker for breast carcinoma. Li, Wan-Feng,Li, Si-Jie,Fan, Zhi-Min,Liu, Xiang-Guo,Wang, Xiao-Ping,Yang, Shuo,Zhou, Xin,Hao, Dong-Yun,Hao, Dong-Yun,Li, Xiang-Jun. 2010

[10]Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319. Mu, Chun Hua,Zhang, Fa Jun,Li, Wen Cai,Lu, Shou Ping,Meng, Zhao Dong,Liu, Xia,Mu, Chun Hua,Liu, Xia,Yang, Yu,Li, Guang Cun. 2016

[11]Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across embryo and endosperm in Maize (Zea may). Wang, Pengfei,Ma, Chuanxi,Wang, Pengfei,Xia, Han,Zhang, Ye,Zhao, Shuzhen,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Li, Aiqin,Wang, Xingjun. 2015

[12]ZmFKBP20-1 improves the drought and salt tolerance of transformed Arabidopsis. Yu, Yanli,Li, Yanjiao,Zhao, Meng,Li, Wencai,Sun, Qi,Li, Wenlan,Meng, Zhaodong,Jia, Fengjuan,Jia, Fengjuan,Li, Nana. 2017

[13]Effects of pollination-prevention on leaf senescence and post-silking nitrogen accumulation and remobilization in maize hybrids released in the past four decades in China. Guo, Song,Chen, Fanjun,Yuan, Lixing,Mi, Guohua,Guo, Song.

[14]Determination of 16 Mycotoxins in Maize by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry. Li, Xia,Liu, Bin,Wang, Fengen,Ma, Xinfeng,Li, Zengmei,Guo, Dongliang,Wang, Yutao,Deng, Ligang,Zhang, Shuqiu,Wan, Fachun. 2018

[15]Meta-analysis of constitutive QTLs for disease resistance in maize and its synteny conservation in the rice genome. Zhao, L.,Wang, Q. Y.,Liu, H. J.,Zhang, C. X.,Li, X. H.. 2015

[16]Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses. Feng, Shangguo,Yang, Yanjun,Xu, Mingfeng,Wang, Huizhong,Shen, Chenjia,Yue, Runqing,Zhang, Lei. 2015

[17]Silver nanoparticles deteriorate the mutual interaction between maize (Zea mays L.) and arbuscular mycorrhizal fungi: a soil microcosm study. Cao, Jiling,Feng, Youzhi,Lin, Xiangui,Cao, Jiling,Feng, Youzhi,Lin, Xiangui,Cao, Jiling,Feng, Youzhi,Lin, Xiangui,Cao, Jiling,He, Shiying. 2017

[18]The changes of organelle ultrastructure and Ca2+ homeostasis in maize mesophyll cells during the process of drought-induced leaf senescence. Ma, Yuan-Yuan,Guo, Xiu-Lin,Liu, Zi-Hui,Ma, Yuan-Yuan,Shao, Hong-Bo,Shao, Hong-Bo,Liu, Bin-Hui. 2011

[19]Establishment of a core collection for maize germplasm preserved in Chinese National Genebank using geographic distribution and characterization data. Li, Y,Shi, YS,Cao, YS,Wang, TY. 2004

[20]Genome-wide analysis of maize NLP transcription factor family revealed the roles in nitrogen response. Ge, Min,Jiang, Lu,Wang, Yuancong,Lv, Yuanda,Zhou, Ling,Liang, Shuaiqiang,Bao, Huabin,Zhao, Han,Liu, Yuhe. 2018

作者其他论文 更多>>

微信小程序