Molecular characterization and expression profiles of olfactory receptor genes in the parasitic wasp, Microplitis mediator (Hymenoptera: Braconidae)

文献类型： 外文期刊

第一作者： Gu, Shao-Hua

作者： Gu, Shao-Hua;Wang, Shan-Ning;Guo, Yu-Yuan;Zhang, Yong-Jun;Ma, Long;Liu, Ze-Wen;Zhou, Jing-Jiang

作者机构：

关键词： Microplitis mediator;Antennal cDNA library;Olfactory receptor;Trophic interaction;Parasitic wasp

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN： 0022-1910

年卷期： 2014 年 60 卷

页码：

收录情况： SCI

摘要： Olfactory receptors (OR) are believed to fulfil an indispensable role in insects host-seeking, mating and ovipositing. We obtained 4785 high-quality expressed sequencing tags (EST) from the antennal cDNA library of the parasitic wasp Microplitis mediator, a natural enemy of crop pests. After assembling, 786 contigs and 2130 singletons were generated. Using BLAST searches, a number of olfactory-related genes were identified, including ESTs encoding for 25 ORs. 14 full-length OR genes were cloned and their expression profiles in the wasp olfactory organs were quantified by real-time qRT-PCR. The results indicated a diverse distribution between the tissues and genders, yet the majority of OR genes are highly expressed in antennae. Three OR genes (0r2, 0r12 and 0r13) are highly expressed in female antennae, eight OR genes (ORco, Or3, 0r4, 0r5, 0r7, 0r8, 0r9 and On 1) are highly expressed in male antennae. This is the first report on tissue-specific expression of wasp OR genes. Our study provides a foundational knowledge to explore and understand the molecular basis of odorant reception in this parasitic wasp and for the study of trophic interactions of natural enemy. (C) 2013 Elsevier Ltd. All rights reserved.

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