Deep sequencing-based identification of pathogen-specific microRNAs in the plasma of rabbits infected with Schistosoma japonicum
文献类型: 外文期刊
第一作者: Cheng, Guofeng
作者: Cheng, Guofeng;Luo, Rong;Hu, Chao;Cao, Jie;Jin, Youxin
作者机构:
关键词: microRNA;Schistosoma japonicum;deep sequencing;diagnosis;biomarkers
期刊名称:PARASITOLOGY ( 影响因子:3.234; 五年影响因子:3.118 )
ISSN: 0031-1820
年卷期: 2013 年 140 卷 14 期
页码:
收录情况: SCI
摘要: Circulating microRNAs (miRNAs) have received considerable attention as a novel class of biomarkers for the diagnosis of cancer and as signalling molecules in mediating intercellular communication. Schistosomes, the causative agents of schistosomiasis, live in the blood vessels of a mammalian host in the adult stage. In the present study, we characterized schistosome-specific small RNA populations in the plasma of rabbits infected with Schistosoma japonicum (S. japonicum) using a deep sequencing method and then identified five schistosome-specific miRNAs, including four known miRNAs (Bantam, miR-3479, miR-10 and miR-3096), and one novel miRNA (miR-0001, miRBase ID: sja-miR-8185). Four of the five schistosome-specific miRNAs were also detected by real-time RT-PCR in the plasma of S. japonicum-infected mice. In addition, our study indicated that schistosome Argonaute 2/3 may be an excretory-secretory (ES) protein. In summary, our findings are expected to provide useful information for further development of novel biomarkers for the diagnosis of schistosomiasis and also for deeper understanding of the mechanism of host-parasite interaction.
分类号:
- 相关文献
作者其他论文 更多>>
-
HL-TRP channel is required for various repellents for the parthenogenetic Haemaphysalis longicornis
作者:Kuang, Ceyan;Cao, Jie;Zhou, Yongzhi;Zhang, Houshuang;Wang, Yanan;Zhou, Jinlin
关键词:HL-TRP channel; Icaridin; DEET; Cinnamaldehyde; Olfaction
-
Multifunctional Analysis of Agriculture from the Perspective of Tradeoff/Synergy: A Case Study of Sichuan Province in Western China
作者:Liao, Qiang;Chen, Chunyan;Lin, Zhengyu;Liu, Yuanli;Cao, Jie;Shao, Zhouling;Kou, Yaowen
关键词:spatiotemporal pattern; agricultural functions; tradeoff and synergies; cluster analysis; Sichuan Province
-
HL-IR mediates cinnamaldehyde repellency behavior in parthenogenetic Haemaphysalis longicornis
作者:Kuang, Ceyan;Shi, Han;Cao, Jie;Zhou, Yongzhi;Zhang, Houshuang;Wang, Yanan;Zhou, Jinlin
关键词:
-
MicroRNA-34-5p regulates the expression of ecdysteroid receptor (ECR) in the process of salivary gland degeneration of ticks
作者:Hu, Shanming;Wang, Yanan;Zhou, Yongzhi;Cao, Jie;Zhang, Houshuang;Zhou, Jinlin
关键词:Salivary gland degeneration; Apoptosis; miR-34-5p; RhECR
-
Tick HRF-dependent ferroptosis pathway to promote tick acquisition of Babesia microti
作者:Chen, Songqin;Hu, Shanming;Zhou, Yongzhi;Cao, Jie;Zhang, Houshuang;Wang, Yanan;Zhou, Jinlin
关键词:Babesia microti; ferroptosis; ticks; HRF; infection
-
Transcriptome reveals the roles and potential mechanisms of CeRNA in the regulation of salivary gland development in the tick Rhipicephalus haemaphysaloides
作者:Hu, Shanming;Chen, Songqin;Wang, Yanan;Zhou, Yongzhi;Cao, Jie;Zhang, Houshuang;Zhou, Jinlin;Zhu, Haotian
关键词:salivary gland; ceRNA; tick; miRNA; lncRNA
-
Ferritin1-mediated ferroptosis participates in granular acini degeneration of Haemaphysalis longicornis salivary glands
作者:Yuan, Dongqi;Chen, Songqin;Zhou, Yongzhi;Cao, Jie;Zhang, Houshuang;Wang, Yanan;Zhou, Jinlin
关键词:Tick; Ferritin 1; Salivary gland degeneration; Ferroptosis