Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry
文献类型: 外文期刊
第一作者: Wen, Zhiyuan
作者: Wen, Zhiyuan;Zhao, Bolin;Song, Kun;Hu, Xule;Chen, Weiye;Kong, Dongni;Ge, Jinying;Bu, Zhigao
作者机构:
关键词: Recombinant Newcastle disease virus;Ebola virus;Glycoprotein;Virus entry;Macropinocytosis
期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )
ISSN: 1743-422X
年卷期: 2013 年 10 卷
页码:
收录情况: SCI
摘要: Background: Using reverse genetics, we generated a recombinant low-pathogenic LaSota strain Newcastle disease virus (NDV) expressing the glycoprotein (GP) of Ebola virus (EBOV), designated rLa-EBOVGP, and evaluated its biological characteristic in vivo and in vitro. Results: The introduction and expression of the EBOV GP gene did not increase the virulence of the NDV vector in poultry or mice. EBOV GP was incorporated into the particle of the vector virus and the recombinant virus rLa-EBOVGP infected cells and spread within them independently of exogenous trypsin. rLa-EBOVGP is more resistant to NDV antiserum than the vector NDV and is moderately sensitive to EBOV GP antiserum. More importantly, infection with rLa-EBOVGP was markedly inhibited by IPA3, indicating that rLa-EBOVGP uses macropinocytosis as the major internalization pathway for cell entry. Conclusions: The results demonstrate that EBOV GP in recombinant NDV particles functions independently to mediate the viral infection of the host cells and alters the cell-entry pathway.
分类号:
- 相关文献
作者其他论文 更多>>
-
Establishment of an immunoperoxidase monolayer assay for the detection of African swine fever virus antibodies
作者:Yin, Li;Wang, Wan;Liu, Fan;Zhang, Zhenjiang;Tesfagaber, Weldu;Liu, Renqiang;Li, Fang;Bu, Zhigao;Zhu, Yuanmao;Zhao, Dongming;Zhao, Dongming
关键词:African swine fever virus; Cell-adapted strain; Wild boar kidney cells; IPMA; Antibody; Serological diagnosis
-
Susceptibility of Ferret and Cat to Porcine Deltacoronavirus: Evidence of Infection in Ferrets But Not Cats
作者:Meng, Shuhuai;Liu, Renqiang;Shuai, Lei;Guo, Huijuan;Dang, Yijing;Bu, Zhigao;Wen, Zhiyuan;Zhang, Hao;Hu, Guangli;Cao, Yongchang;Bu, Zhigao
关键词:cats; ferrets; interspecies transmission; porcine deltacoronavirus (PDCoV)
-
Rabies virus utilizes neuropilin 2 as an endocytic receptor to trigger TGFBR1-mediated actin polymerization
作者:Sun, Ziruo;Wang, Jinqiu;Wen, Zhiyuan;Shuai, Lei;Sun, Wenjing;Yang, Mengjie;Wang, Jinyu;Chen, Junyu;Ge, Jinying;Chen, Weiye;Wang, Xijun;Bu, Zhigao;Wang, Jinliang;Bu, Zhigao
关键词:rabies virus; neuropilin 2; receptor; transforming growth factor-beta receptor I; actin polymerization
-
Virtual Screening and Molecular Dynamics Simulation Targeting the ATP Domain of African Swine Fever Virus Type II DNA Topoisomerase
作者:Zhao, Rui;Zhao, Dongming;Zhao, Rui;Tesfagaber, Weldu;Song, Linfei;Zhang, Zhenjiang;Li, Fang;Bu, Zhigao;Zhao, Dongming;Hou, Lezi
关键词:ASFV; type II DNA topoisomerase; ATPase domain; small molecule inhibitors; virtual screening
-
Rabies virus-based oral and inactivated vaccines protect minks against SARS-CoV-2 infection and transmission
作者:Huo, Hong;Xiao, Shuang;Wang, Jinming;Wang, Xijun;Ge, Jinying;Zhong, Gongxun;Wen, Zhiyuan;Wang, Chong;Wang, Jinliang;Wang, Han;Shuai, Lei;Bu, Zhigao;Huo, Hong;Zhong, Gongxun;Wen, Zhiyuan;Wang, Chong;Wang, Jinliang;He, Xijun;Shuai, Lei;Bu, Zhigao;Bu, Zhigao
关键词:SARS-CoV-2; rabies virus; oral vaccine; inactivated vaccine; mink
-
H5N1 virus invades the mammary glands of dairy cattle through 'mouth-to-teat' transmission
作者:Shi, Jianzhong;Kong, Huihui;Cui, Pengfei;Deng, Guohua;Zeng, Xianying;Jiang, Yongping;Chen, Lei;Zhuang, Yichao;Wang, Yan;Ma, Jinming;Li, Jiaqi;Zhang, Yaping;Wang, Congcong;He, Chen;Yang, Jiaxin;Li, Jiongjie;Liu, Wenyu;Liu, Jinxiong;Chen, Pucheng;Tian, Guobin;Li, Chengjun;Bu, Zhigao;Chen, Hualan;He, Xijun;Zhang, Xianfeng;Guan, Yuntao;Bu, Zhigao;Yang, Jinyu;Mao, Shenggang
关键词:dairy cattle; replication; transmission; vaccination
-
An attenuated African swine fever virus expressing the E2 glycoprotein of classical swine fever virus protects pigs against challenge of both viruses
作者:Zhang, Jiwen;Li, Fang;Chen, Weiye;Li, Yongfeng;Zhang, Zhenjiang;Hua, Ronghong;Liu, Renqiang;Zhu, Yuanmao;Sun, Encheng;Qiu, Huaji;Bu, Zhigao;Zhao, Dongming;Sun, Encheng
关键词:African swine fever virus; classical swine fever virus; glycoprotein E2; protection; bivalent vaccine