Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry
文献类型: 外文期刊
第一作者: Wen, Zhiyuan
作者: Wen, Zhiyuan;Zhao, Bolin;Song, Kun;Hu, Xule;Chen, Weiye;Kong, Dongni;Ge, Jinying;Bu, Zhigao
作者机构:
关键词: Recombinant Newcastle disease virus;Ebola virus;Glycoprotein;Virus entry;Macropinocytosis
期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )
ISSN: 1743-422X
年卷期: 2013 年 10 卷
页码:
收录情况: SCI
摘要: Background: Using reverse genetics, we generated a recombinant low-pathogenic LaSota strain Newcastle disease virus (NDV) expressing the glycoprotein (GP) of Ebola virus (EBOV), designated rLa-EBOVGP, and evaluated its biological characteristic in vivo and in vitro. Results: The introduction and expression of the EBOV GP gene did not increase the virulence of the NDV vector in poultry or mice. EBOV GP was incorporated into the particle of the vector virus and the recombinant virus rLa-EBOVGP infected cells and spread within them independently of exogenous trypsin. rLa-EBOVGP is more resistant to NDV antiserum than the vector NDV and is moderately sensitive to EBOV GP antiserum. More importantly, infection with rLa-EBOVGP was markedly inhibited by IPA3, indicating that rLa-EBOVGP uses macropinocytosis as the major internalization pathway for cell entry. Conclusions: The results demonstrate that EBOV GP in recombinant NDV particles functions independently to mediate the viral infection of the host cells and alters the cell-entry pathway.
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