A Sensitive Immunoaffinity Column-Linked Indirect Competitive ELISA for Ochratoxin A in Cereal and Oil Products Based on a New Monoclonal Antibody
文献类型: 外文期刊
第一作者: Li, Xin
作者: Li, Xin;Li, Peiwu;Zhang, Qi;Zhang, Zhaowei;Li, Ran;Zhang, Wen;Ding, Xiaoxia;Chen, Xiaomei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Zhaowei;Li, Ran;Li, Peiwu;Zhang, Qi;Zhang, Zhaowei;Li, Ran;Ding, Xiaoxia;Li, Xin;Li, Peiwu;Zhang, Wen;Chen, Xiaomei;Tang, Xiaoqian
作者机构:
关键词: Ochratoxin A;Monoclonal antibody;Immunoaffinity column-linked indirect competitive ELISA;Cereal;Oil products
期刊名称:FOOD ANALYTICAL METHODS ( 影响因子:3.366; 五年影响因子:3.07 )
ISSN: 1936-9751
年卷期: 2013 年 6 卷 5 期
页码:
收录情况: SCI
摘要: A new sensitive monoclonal antibody (mAb) 1H2 against ochratoxin A (OTA) was reported herein. This mAb belonged to the immunoglobulin G1 (k chain) isotype. In the optimized indirect competitive enzyme-linked immunosorbent assay (icELISA), 1H2 showed a 50 % inhibition concentration (IC50) value of 0.058 ng/mL and a detection limit (IC10) of 0.001 ng/mL. The cross-reactivity of 1H2 with ochratoxin B, aflatoxins, deoxynivalenol, zearalenone, T-2 toxin, or fumonisins was below 0.3 %. Based on this mAb, an immunoaffinity column (IAC)-linked icELISA was developed for OTA detection in the cereal and oil products. The working range of the assay for solid sample was 0.36-16 mu g/kg. The recoveries from spiked samples of IAC-linked icELISA ranged from 83 to 101 %. These recoveries were much higher than those of icELISA (21-78 %) and in good agreement with those obtained by using the standard high-performance liquid chromatography method (87-110 %). The results indicated that the mAb 1H2 had the values for studies of OTA in the crude agricultural products.
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