Isolation and characterization of mesenchymal stem cells from chicken bone marrow

文献类型： 外文期刊

第一作者： Bai, Chunyu

作者： Bai, Chunyu;Ma, Yuehui;Chen, Lina;Guan, Weijun;Bai, Chunyu;Zhang, Minghai;Hou, Lingling

作者机构：

关键词： Chicken;Bone marrow;Mesenchymal stem cells;Biological characteristics

期刊名称：CELL AND TISSUE BANKING （ 影响因子：1.522； 五年影响因子：1.968 ）

ISSN： 1389-9333

年卷期： 2013 年 14 卷 3 期

页码：

收录情况： SCI

摘要： The bone marrow mesenchymal stem cells (BMSCs) are multipotent stem cells, which can differentiate in vitro into many cell types. However, the vast majority of experimental materials were obtained from human, mouse, rabbit and other mammals, but rarely in poultry. So, in this study, Thirty- to sixty-day old chicken was chosen as experimental animal, to isolate and characterize BMSCs from them. To investigate the biological characteristics of chicken BMSCs, immunofluorescence and RT-PCR were used to detect the characteristic surface markers of BMSCs. Growth curves were drawn in accordance with cell numbers. To assess the differentiation capacity of the BMSCs, cells were induced to differentiate into osteoblasts, adipocytes, and endothelial cells. The surface markers of BMSCs, CD29, CD44, CD31, CD34, CD71 and CD73, were detected by immunofluorescence and RT-PCR assays. The growth curves of different passages were all typically sigmoidal. Karyotype analysis showed that these in vitro cultured cells were genetically stable. In addition, BMSCs were successfully induced to differentiate into osteoblasts, adipocytes, and endothelial cells. The results suggest that the BMSCs isolated from chicken possess similar biological characteristics with those separated from other species, and their multi-lineage differentiation potentiality herald a probable application for cellular transplant therapy in tissue engineering.

分类号：

  • 相关文献

[1]Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells. Bai, Chunyu,Gao, Yuhua,Li, Qian,Feng, Yuan,Yu, Yanze,Meng, Gentong,Zhang, Minghai,Bai, Chunyu,Gao, Yuhua,Li, Qian,Guan, Weijun.

[2]Cultivation and Biological Characterization of Chicken Primordial Germ Cells. Ji, Meng,Guan, Wei-jun,Gao, Yu-hua,Bai, Chun-yu,Ma, Yue-hui,Li, Xiang-chen,Li, Lu. 2016

[3]Characterization of vascular endothelial progenitor cells from chicken bone marrow. Bai, Chunyu,Pu, Yabin,Guan, Weijun,Ma, Yuehui,Hou, Lingling,Bai, Chunyu,Zhang, Minghai. 2012

[4]Anemia induced by repeated exposure to cyanobacterial extracts with explorations of underlying mechanisms. Zhang, Xuezhen,Xie, Ping,Li, Dapeng,Wang, Jun,Yuan, Gailing,Zhao, Yanyan,Tang, Rong,Xie, Ping,Shi, Zechao.

[5]MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells. Bai, Chunyu,Gao, Yuhua,Li, Xiangchen,Wang, Kunfu,Xiong, Hui,Shan, Zhiqiang,Zhang, Ping,Wang, Wenjie,Guan, Weijun,Ma, Yuehui. 2017

[6]Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration. Li, Yang,Liu, Ming-yuan,Wang, Xue-lin,Han, Wen-yu,Liu, Ya-hui,Suo, Jian,Li, Zhi-jie,Li, Ya-gang,Jin, Hu. 2013

[7]Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous Mats. Luo, Yu,Shen, He,Cao, Yuhua,Huang, Jie,Zhang, Mengxin,Dai, Jianwu,Zhang, Zhijun,Luo, Yu,Shi, Xiangyang,Fang, Yongxiang,Dai, Jianwu,Fang, Yongxiang. 2015

[8]MicroRNA-34c acts as a bidirectional switch in the maturation of insulin-producing cells derived from mesenchymal stem cells. Bai, Chunyu,Yang, Wancai,Gao, Yuhua,Zhang, Xiangyang,Bai, Chunyu,Gao, Yuhua,Guan, Weijun,Yang, Wancai. 2017

[9]Wip1 knockout inhibits the proliferation and enhances the migration of bone marrow mesenchymal stem cells. Tang, Yiting,Sheng, Ming,Liu, Honglin,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Xiong, Kai. 2015

[10]Biological characterization of mesenchymal stem cells from bovine umbilical cord. Xiong, Hui,Bai, Chunyu,Wu, Shuang,Gao, Yuhua,Lu, Taofeng,Guan, Weijun,Ma, Yuehui,Xiong, Hui,Hu, Qingyun.

[11]Differentiation potential of bone marrow mesenchymal stem cells in duck. Li, Linfeng,Gong, Xuelian,Liu, Hongkun,Chen, Lina,Guan, Weijun,Ma, Yuehui,Li, Linfeng,Bai, Xiujuan. 2009

[12]Inhibitory effect and mechanism of mesenchymal stem cells on liver cancer cells. Hou, Lingling,Wang, Xiaoyu,Zhou, Yaqiong,Ma, Haibin,Wang, Ziling,He, Jinsheng,Hu, Honggang,Guan, Weijun,Ma, Yuehui. 2014

[13]Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton. Zhang, Ya-Lin,Li, Zhi-Fang,Feng, Zi-Li,Feng, Hong-Jie,Zhao, Li-Hong,Shi, Yong-Qiang,Hu, Xiao-Ping,Zhu, He-Qin.

[14]Establishment of Big Bone chicken fibroblast cell bank and study of its biological characteristics. Su, Xiaohua,Jin, Dapeng,Yue, Hua,Pu, Yabin,Ma, Yuehui,Guan, Weijun,Su, Xiaohua,Li, Chunying,Yue, Hua,Jin, Dapeng. 2011

[15]Biological characterization of sheep kidney-derived mesenchymal stem cells. Ji, Meng,Bai, Chunyu,Fan, Ya'nan,Ma, Caiyun,Li, Xiangchen,Guan, Weijun,Li, Lu. 2016

[16]Sensitivity to fluopicolide of wild type isolates and biological characteristics of fluopicolide-resistant mutants in Pseudoperonospora cubensis. Wang, Wenqiao,Yan, Lei,Meng, Runjie,Zhao, Jianjiang,Zhang, Xiaofeng,Han, Xiuying,Ma, Zhiqiang. 2014

[17]Establishment and Biological Characteristic Research of Yuxizhiwei sheep Fibroblast Cell Bank. Wang, Hui,Li, Xiangchen,Li, Changli,Zhang, Wenxiu,Guan, Weijun,Ma, Yuehui,Wang, Hui. 2012

[18]Establishment and characterization of a Chinese Game chicken embryo fibroblast bank. Li, Xiangchen,Bai, Chunyu,Su, Xiaohua,Jin, Dapeng,Zhao, Qianjun,He, Xiaohong,Guan, Weijun,Ma, Yuehui,Bai, Chunyu,Jin, Dapeng,Su, Xiaohua.

[19]Establishment and biological characteristics of Ujumqin sheep fibroblast line. Na, Ri Su,Zhao, Qian Jun,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei,Guan, Wei Jun,Ma, Yue Hui,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei.

[20]Establishment and biological characteristics of Qingyuan partridge chicken fibroblast line. Na, R. S.,Bai, C. Y.,Jin, D. P.,Su, X. H.,Feng, B. G.,Guan, W. J.,Ma, Y. H.,Jin, D. P.,Su, X. H.,Feng, B. G..

作者其他论文 更多>>

微信小程序