A SOE-PCR method of introducing multiple mutations into Mycoplasma gallisepticum neuraminidase

文献类型： 外文期刊

第一作者： Tan, Lei

作者： Tan, Lei;Chen, Hongjun;Yu, Shengqing;Qiu, Xusheng;Song, Cuiping;Chen, Danqing;Zhang, Shilei;Zhang, Fanqing;He, Suibin;Shen, Xinyue;Hu, Meirong;Ding, Chan;Shen, Xinyue

作者机构：

关键词： M. gallisepticum;SOE-PCR;MGA_0329 gene;Neuraminidase

期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）

ISSN： 0167-7012

年卷期： 2013 年 94 卷 2 期

页码：

收录情况： SCI

摘要： A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes. (C) 2013 Elsevier B.V. All rights reserved.

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