Enrichment process for alpha-linolenic acid from silkworm pupae oil

文献类型： 外文期刊

第一作者： Wang, Jun

作者： Wang, Jun;Zhang, Jin-Liang;Wu, Fu-An;Wu, Fu-An

作者机构：

关键词： alpha-Linolenic acid;beta-Cyclodextrin inclusion;Purification;Silkworm pupae oil;Urea complexation

期刊名称：EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY （ 影响因子：2.679； 五年影响因子：2.627 ）

ISSN： 1438-7697

年卷期： 2013 年 115 卷 7 期

页码：

收录情况： SCI

摘要： The desilked silkworm (Bombyx mori L.) pupae oil is a good source of -linolenic acid (ALA), but selectively enriching ALA is a great technical challenge due to the presence of multiple analogues such as linoleic acid and oleic acid. To set up a simple, highly selective and low-cost combinative inclusion process for obtaining ALA concentrate from silkworm pupae oil, the effects of -cyclodextrin (-CD) inclusion parameters on the purity and recovery of polyunsaturated fatty acid (PUFA) were investigated; then, four types of combinative processes using -CD and/or urea were compared. The results indicated that a two-stage combinative inclusion process was established. In the first stage, when the volume ratio of -CD-saturated solution to free fatty acids ethanol solution was 9 v/v, the dispersion temperature and time were 60 degrees C and 1.5h, the freezing temperature and time were -10 degrees C and 15h, and the purity of ALA was raised from 15.0 to 47.6% with a recovery of 39.4%. Then, the purity of ALA was further increased to 67.4% in the second-stage operation with a corresponding recovery of 34.8% using urea complexation.

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