Lactobacillus planarum subsp. plantarum JCM 1149 vs. Aeromonas hydrophila NJ-1 in the anterior intestine and posterior intestine of hybrid tilapia Oreochromis niloticus female x Oreochromis aureus male: An ex vivo study

文献类型: 外文期刊

第一作者: Ren, Pengfei

作者: Ren, Pengfei;Xu, Li;Yang, Yaling;He, Suxu;Liu, Wenshu;Zhou, Zhigang;Ringo, Einar

作者机构:

关键词: Tilapia;Colonization;Immunity;Microbiology

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

ISSN: 1050-4648

年卷期: 2013 年 35 卷 1 期

页码:

收录情况: SCI

摘要: To investigate the ex vivo interactions of probiotic-pathogen-host in warm-water fish, hybrid tilapia (Oreochromis niloticus female x Oreochromis aureusd male) were sacrificed to isolate anterior and posterior intestine for incubation with phosphate-buffered saline (PBS; pH 7.2) as the control, Lactobacillus plantarum JCM 1149 at 1.0 x 10(9) CPU/ml, Aeromonas hydrophila NJ-1 at 1.0 x 10(8) CPU/ml, or the both combination. Denaturing Gradient Gel Electrophoresis (DGGE) fingerprint and consequent sequence analysis confirmed anterior intestine sac was more prone to the colonization of L plantarum JCM 1149 and A. hydrophila NJ-1 than the posterior part. L plantarum JCM 1149 and A. hydrophila NJ-1 inhibited the population each other in anterior or posterior sac, indicating their competition for the colonization. The induced expression of HSP70, IL-beta and TNF-alpha in the anterior sac by the addition of L plantarum JCM 1149 or A. hydrophila NJ-1 demonstrated the activity and a local immune response of ex vivo anterior sac. Compared with posterior intestine, higher population colonization and more sensitive immune response of anterior sac indicated differential patterns for the probiotic-pathogen-host interactions. Scanning electronic microscopy (SEM) observation showed that pathogen A. hydrophila NJ-1 damaged the anterior intestine, which was alleviated by the pretreatment of L plantarum JCM 1149, showing its probiotic effect. (C) 2013 Elsevier Ltd. All rights reserved.

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