Mesorhizobium qingshengii sp nov., isolated from effective nodules of Astragalus sinicus

文献类型： 外文期刊

第一作者： Zheng, Wen Tao

作者： Zheng, Wen Tao;Li, Ying, Jr.;Wang, Rui;Sui, Xin Hua;Zhang, Jun Jie;Wang, En Tao;Chen, Wen Xin;Zhang, Xiao Xia;Wang, En Tao

作者机构：

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2013 年 63 卷

页码：

收录情况： SCI

摘要： In a study on the diversity of rhizobia isolated from root nodules of Astragalus sinicus, five strains showed identical 16S rRNA gene sequences. They were related most closely to the type strains of Mesorhizobium loti, Mesorhizobium shangrilense, Mesorhizobium ciceri and Mesorhizobium australicum, with sequence similarities of 99.6-99.8%. A polyphasic approach, including 16S-23S intergenic spacer (IGS) RFLP, comparative sequence analysis of 16S rRNA, atpD, glnll and recA genes, DNA-DNA hybridization and phenotypic tests, clustered the five isolates into a coherent group distinct from all recognized Mesorhizobium species. Except for strain CCBAU 33446, from which no symbiotic gene was detected, the four remaining strains shared identical nifH and nodC gene sequences and nodulated with Astragalus sinicus. In addition, these five strains showed similar but different fingerprints in IGS-RFLP and BOX-repeat-based PCR, indicating that they were not clones of the same strain. They were also distinguished from recognized Mesorhizobium species by several phenotypic features and fatty acid profiles. Based upon all the results, we suggest that the five strains represent a novel species for which the name Mesorhizobium qingshengii sp. nov. is proposed. The type strain is CCBAU 33460(T) (=CGMCC 1.12097(T)=LMG 26793(T)=HAMBI 3277(T)). The DNA G+C content of the type strain is 59.52 mol% (T-m).

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序