Decreased saturated fatty acids, total cholesterol and LDL-C in sdd17 mice

文献类型: 外文期刊

第一作者: Chen, Yong-Gang

作者: Chen, Yong-Gang;Yan, Bo-Wen;Gou, Ke-Mian;Cao, Wen-Guang;Ma, Shu-Yu

作者机构:

关键词: Desaturase;sdd17;Transgenic;Mouse;Fatty Acids;Cholesterol;Lipoprotein

期刊名称:FRONTIERS IN BIOSCIENCE-LANDMARK ( 影响因子:4.009; 五年影响因子:3.927 )

ISSN: 1093-9946

年卷期: 2013 年 18 卷

页码:

收录情况: SCI

摘要: SDD17, a delta-15 desaturase from the fungus Saprolegnia can convert arachidonic acid to eicosapentanoic acid in yeast, plant embryos, and mammalian cells. Here, we generated transgenic mice that carried two copies of codon-optimized sdd17 cDNA within a non-coding domain of chromosome 6. RT-PCR analysis revealed that the foreign gene was expressed in the transgenic tissues. Gas chromatography showed that the levels of total unsaturated fatty acids in muscle, liver, and spleen tissues were significantly (p<0.05) increased in transgenic mice compared to non-transgenic mice at 3 or 8 weeks of age. In addition, the serum concentrations of total cholesterol and low-density lipoprotein cholesterol in transgenic females, but not in males, were significantly lower than those in sex-matched non-transgenic mice. These results suggest that endogenous sdd17 expression is beneficial for mammalian health and that its effects on fatty acid profiles may differ between sexes.

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