Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice

文献类型： 外文期刊

第一作者： Yin, Yan-hui

作者： Yin, Yan-hui;Li, Bi-chun;Wei, Guang-hui;Zhu, Cai-ye;Li, Wei;Zhang, Ya-ni;Du, Li-xin;Cao, Wen-guang

作者机构：

关键词： amino acids;complementary DNA;fusion proteins;gene expression;genes;nucleotide sequences;reverse transcriptase PCR;transgenic animals;transgenics;goats;mice;Capra;Bovidae;ruminants;Artiodactyla;ungulates;mammals;vertebrates;Chordata;animals;eukaryotes;Muridae;rodents;cDNA;DNA sequences;genetically engineered animals;genetically modified animals;GMOs;reverse transcriptase polymerase chain reaction;RT-PCR;Xuhuai (goat breed)

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to clone the heart-type fatty acid binding protein (H-FABP) gene of Xuhuai goat, to explore it bioinformatically, and analyze the subcellular localization using enhanced green fluorescent protein (EGFP). The results showed that the coding sequence (CDS) length of Xuhuai goat H-FABP gene was 402 bp, encoding 133 amino acids (GenBank accession number AY466498.1). The H-FABP cDNA coding sequence was compared with the corresponding region of human, chicken, brown rat, cow, wild boar, donkey, and zebrafish. The similarity were 89%, 76%, 85%, 84%, 93%, 91%, 70%, respectively. For the corresponding amino acid sequences, the similarity were 90%, 79%, 88%, 97%, 95%, 94%, 72%, respectively. This study did not find the signal peptide region in the H-FABP protein; it revealed that H-FABP protein might be a nonsecreted protein. H-FABP expression was detected in vitro by reverse transcription-polymerase chain reaction (RT-PCR), and the EGFP-H-FABP fusion protein was localized to the cytoplasm. The gene could also be transiently and permanently expressed in mice.

分类号： Q343

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