Detection and new genetic environment of the pleuromutilinlincosamidestreptogramin A resistance gene lsa(E) in methicillin-resistant Staphylococcus aureus of swine origin

文献类型: 外文期刊

第一作者: Li, Beibei

作者: Li, Beibei;Yao, Jiannan;Shi, Zixue;Wei, Jianchao;Shao, Donghua;Wang, Shaohui;Ma, Zhiyong;Wendlandt, Sarah;Schwarz, Stefan;Liu, Yiqiu;Zhang, Qing

作者机构:

关键词: multidrug resistance;ABC transporters;MRSA;plasmids

期刊名称:JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY ( 影响因子:5.79; 五年影响因子:5.851 )

ISSN: 0305-7453

年卷期: 2013 年 68 卷 6 期

页码:

收录情况: SCI

摘要: To investigate the genetic basis of pleuromutilin resistance in porcine methicillin-resistant Staphylococcus aureus (MRSA) and to map the genetic environment of the identified plasmid-borne resistance gene. Seventy porcine MRSA isolates, which exhibited high MICs of tiamulin, valnemulin and retapamulin, were investigated for pleuromutilin resistance genes and mutations. They were characterized by staphylococcal cassette chromosome mec (SCCmec) typing, spa typing and multilocus sequence typing (MLST). Plasmid DNA was extracted from the lsa(E)-positive strains and transferred to S. aureus RN4220 for selection of resistance plasmids. The plasmid-borne lsa(E) gene region was sequenced and 10 overlapping PCR assays for the analysis of the genetic environment of lsa(E) were developed. All 70 MRSA isolates were ST9 (MLST)-t899 (spa)-IVa (SCCmec). Sixteen isolates carried the lsa(E) gene; all others were negative for known pleuromutilin resistance mechanisms. An lsa(E)-carrying plasmid of 41 kb was detected in a single isolate. Sequence analysis revealed that the lsa(E) gene was located in a multiresistance gene cluster, which showed partial homology to clusters identified in MRSA, methicillin-susceptible S. aureus (MSSA) and Enterococcus faecalis. PCR analysis of the remaining isolates revealed a partly deleted multiresistance gene cluster in 6/15 isolates and solely the lsa(E) gene without the known flanking regions in 9/15 isolates. We identified the pleuromutilinlincosamidestreptogramin A resistance gene lsa(E) in porcine MRSA isolates. The multiresistance gene cluster in which lsa(E) was located differed from the previously described ones found in human MRSA/MSSA or in E. faecalis. The location of lsa(E) on a multiresistance plasmid facilitates its persistence and dissemination.

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