Minimalist nanobody architecture: A double-edged sword for immunological analysis

文献类型： 外文期刊

第一作者： Wang, Yulong

作者： Wang, Yulong;Liu, Pengyan;Zhang, Yaodong;Zhang, Cunzheng;Wang, Yulong;Luo, Ruifeng;Yang, Huijuan;Dong, Sa;Hua, Xiude;Hammock, Bruce D.

作者机构：

关键词： Nanobody-based immunoassay; Miniaturized structure; Small size; Bioconjugation chemistry

期刊名称：TRAC-TRENDS IN ANALYTICAL CHEMISTRY （ 影响因子：12.0； 五年影响因子：13.2 ）

ISSN： 0165-9936

年卷期： 2025 年 192 卷

页码：

收录情况： SCI

摘要： A little over three decades developing, nanobodies (Nbs) are gaining increasing acceptance as analytical tools and promising therapeutic agents utilized within biological applications. They are well expressed in various microorganisms, easy to engineer, and highly robust to avoid storage deterioration and batch variance risks compared to that of conventional immunoglobulin G (IgG) antibodies, showing great promise as a new generation of commercial immunoreagents for analytical purpose. Nbs have remarkable characteristics that make them more favorable for use than conventional IgG antibodies or fragments thereof in certain areas of research. However, what is not widely appreciated is the two sides of their miniaturized structure when developing analytical methods. The progress made on advantages and limitations of miniaturized structure of Nb utilized in immunological analysis is reviewed in detail here. The goal is to offer the reader with (1) how Nb size enhances analytical performance; (2) in which scenarios limitations occur; and (3) how to mitigate these limitations. We focus in particular on the immobilization and bioconjugation chemistry to further amplify size contribution to analytical performance, as well as to compensate performance degradation results from miniaturized structure of Nbs.

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