Clone and identification of bifunctional Delta 12/Delta 15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae

文献类型: 外文期刊

第一作者: Zhang Yan

作者: Zhang Yan;Liang Zhuo;Jiang Mulan;Wan Xia;Gon Yangmin;Zhang Yinbo;Huang Fenghong;Zhang Yan;Huang Fenghong

作者机构:

关键词: Lipomyces kononenkoae;fatty acid desaturase;polyunsaturated fatty acid;alpha-linolenic acid;linoleic acid

期刊名称:FOOD SCIENCE AND BIOTECHNOLOGY ( 影响因子:2.391; 五年影响因子:2.108 )

ISSN: 1226-7708

年卷期: 2013 年 22 卷 2 期

页码:

收录情况: SCI

摘要: In modern diet of human being, the omega-6/omega-3 ratio of dietary fatty acid was shifted much towards omega-6 series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Delta 12/Delta 15 bifunctional FAD which could not only produce linoleic acid and alpha-linolenic acid use just oleic acid as substrate but also adjust the omega- 6/omega-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.

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