Clone and identification of bifunctional Delta 12/Delta 15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae
文献类型: 外文期刊
第一作者: Zhang Yan
作者: Zhang Yan;Liang Zhuo;Jiang Mulan;Wan Xia;Gon Yangmin;Zhang Yinbo;Huang Fenghong;Zhang Yan;Huang Fenghong
作者机构:
关键词: Lipomyces kononenkoae;fatty acid desaturase;polyunsaturated fatty acid;alpha-linolenic acid;linoleic acid
期刊名称:FOOD SCIENCE AND BIOTECHNOLOGY ( 影响因子:2.391; 五年影响因子:2.108 )
ISSN: 1226-7708
年卷期: 2013 年 22 卷 2 期
页码:
收录情况: SCI
摘要: In modern diet of human being, the omega-6/omega-3 ratio of dietary fatty acid was shifted much towards omega-6 series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Delta 12/Delta 15 bifunctional FAD which could not only produce linoleic acid and alpha-linolenic acid use just oleic acid as substrate but also adjust the omega- 6/omega-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.
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