Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells

文献类型： 外文期刊

第一作者： Wang, Yan

作者： Wang, Yan;Cui, Haixin;Sun, Changjiao;Du, Wei;Cui, Jinhui;Zhao, Xiang

作者机构：

关键词： magnetic;fluorescent nanoparticles;gene carrier;location

期刊名称：NANOSCALE RESEARCH LETTERS （ 影响因子：4.703； 五年影响因子：4.193 ）

ISSN： 1931-7573

年卷期： 2013 年 8 卷

页码：

收录情况： SCI

摘要： We evaluated the performance of green fluorescent magnetic Fe3O4 nanoparticles (NPs) as gene carrier and location in pig kidney cells. When the mass ratio of NPs to green fluorescent protein plasmid DNA reached 1:16 or above, DNA molecules can be combined completely with NPs, which indicates that the NPs have good ability to bind negative DNA. Atomic force microscopy (AFM) experiments were carried out to investigate the binding mechanism between NPs and DNA. AFM images show that individual DNA strands come off of larger pieces of netlike agglomerations and several spherical nanoparticles are attached to each individual DNA strand and interact with each other. The pig kidney cells were labelled with membrane-specific red fluorescent dye 1,1(')-dioctadecyl-3,3,3('),3(')-tetramethylindocarbocyanine perchlorate and nucleus-specific blue fluorescent dye 4('),6-diamidino-2-phenylindole dihydrochloride. We found that green fluorescent nanoparticles can past the cell membrane and spread throughout the interior of the cell. The NPs seem to locate more frequently in the cytoplasm than in the nucleus.

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