文献类型： 外文期刊

第一作者： Joung, Young Hee

作者： Joung, Young Hee;Hyun, Jung Oh;Lee, Do Hyung;Suh, Jeung Keun;Hill, Jerry L.;Roh, Mark S.;Mu, Ding;Luo, Juchun;Kawahara, Takayuki

作者机构：

关键词： P. sylvestris var. sylvestriformis;chloroplast DNA;simple sequence repeat sequencing;hybrid swarm population

期刊名称：JOURNAL OF FORESTRY RESEARCH （ 影响因子：2.149； 五年影响因子：1.877 ）

ISSN： 1007-662X

年卷期： 2013 年 24 卷 1 期

页码：

收录情况： SCI

摘要： To confirm a hybrid swarm population of Pinus densiflora x P. sylvestris in Jilin, China, we used needles and seeds from P. densiflora, P. sylvestris, and P. densiflora x P. sylvestris collected from natural stands or experimental stations to study whether shoot apex morphology of 4-year old seedlings can be correlated with the sequence of a chloroplast DNA simple sequence repeat marker (cpDNA SSRs). Total genomic DNA was extracted and subjected to sequence analysis of the pine cpDNA SSR marker Pt15169. Results show that morphological characters from 4-year old seedlings did not correlate with sequence variants of this marker. Marker haplotypes from all P. sylvestris trees had a CTAT element that was absent from all sampled P. densiflora trees. However, both haplotype classes involving this insertion/deletion element were found in a P. densiflora x P. sylvestris population and its seedling progeny. It was concluded that the P. densiflora x P. sylvestris accessions sampled from Jilin, China resulted from bi-directional crosses, as evidenced by both species' cpDNA haplotypes within the hybrid swarm population.

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