Rapid detection and identification of viroids in the genus Coleviroid using a universal probe

文献类型： 外文期刊

第一作者： Wu, Zujian

作者： Wu, Zujian;Xie, Lianhui;Jiang, Dongmei;Kang, Ni;Qin, Lu;Li, Shifang;Hou, Wanying;Sano, Teruo

作者机构：

关键词： Coleviroid;CCR;Hybridization;Coleus;CbVd

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2013 年 187 卷 2 期

页码：

收录情况： SCI

摘要： A simple, low-cost hybridization assay using a universal DIG-labeled riboprobe for the rapid detection and identification of coleus viroids is presented. An octamer of 32-nucleotide sequence derived from the central conserved region (CCR) of viroids in the genus Coleviroid was used to develop a universal cRNA probe (8-central-conserved-region probe, 8CCR probe) for coleus viroids. Dot-blot hybridization assays demonstrated that the sensitivity of this probe was similar to specific probes for each CbVd, and Northern hybridization results revealed that at least four coleus viroids could be distinguished readily and simultaneously using the 8CCR probe. Batch detection assay showed that hybridization using the 8CCR probe can identify coleus viroids rapidly and effectively. This rapid and low-cost molecular hybridization technique is an effective way to survey the occurrence of coleus viroids, and has reference for the detection of other viroids and possibly viruses. (C) 2012 Elsevier B.V. All rights reserved.

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