Detection of mink enteritis virus by loop-mediated isothermal amplification (LAMP)

文献类型： 外文期刊

第一作者： Wang, Jianke

作者： Wang, Jianke;Cheng, Shipeng;Yi, Li;Cheng, Yuening;Yang, Shen;Xu, Hongli;Li, Zhenguang;Shi, Xinchuan;Wu, Hua;Yan, Xijun

作者机构：

关键词： Loop-mediated isothermal amplification (LAMP);Mink enteritis virus

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2013 年 187 卷 2 期

页码：

收录情况： SCI

摘要： Loop-mediated isothermal amPlification (LAMP) method was discovered in the last decade but only used for the first time in the diagnosis of mink enteritis virus (MEV) infection in this study. The amplification could he completed within 60 min, under isothermal condition at 65 degrees C, by employing a set of four primers targeting the VP2 gene of MEV. The LAMP was more sensitive than the conventional PCR, with a detection limit of 10(-1) median tissue culture infective doses (TCID50)/ml per reaction, compared with 10 TCID50/ml for PCR analysis. No cross reactivity was observed for other related viruses, including canine distemper virus (CDV) and Aleutian mink disease parvovirus (AMDV). Eighty four of 230 clinical samples were found to be positive for MEV, which is higher than that determined by using the conventional PCR method (68). The results indicate the LAMP can be potentially used to determine MEV as a simple, rapid procedure. This assay would be an available alternative to PCR analysis for the diagnosis of MEV infection in mink, particularly in less well-equipped laboratories and in rural settings where resources are limited. (C) 2012 Elsevier B.V. All rights reserved.

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