CRISPR/dCas-mediated gene activation toolkit development and its application for parthenogenesis induction in maize

文献类型： 外文期刊

第一作者： Qi, Xiantao

作者： Qi, Xiantao;Gao, Huimin;Lv, Renyao;Mao, Wenbo;Zhu, Jinjie;Liu, Changling;Mao, Long;Li, Xinhai;Xie, Chuanxiao;Qi, Xiantao;Zhu, Jinjie;Li, Xinhai;Xie, Chuanxiao

作者机构：

关键词： CRISPRa; ZmBBM2; egg cell; apomixis engineering; maternal haploid

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 2 期

页码：

收录情况： SCI

摘要： Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems can be engineered as programmable transcription factors to either activate (CRISPRa) or inhibit transcription. Apomixis is extremely valuable for the seed industry in breeding clonal seeds with pure genetic backgrounds. We report here a CRISPR/dCas9-based toolkit equipped with dCas9-VP64 and MS2-p65-HSF1 effectors that may spe-cifically target genes with high activation capability. We explored the application of in vivo CRISPRa target-ing of maize BABY BOOM2 (ZmBBM2), acting as a fertilization checkpoint, as a means to engineer parthe-nogenesis. We detected ZmBBM2 transcripts only in egg cells but not in other maternal gametic cells. Activation of ZmBBM2 in egg cells in vivo caused maternal cell-autonomous parthenogenesis to produce haploid seeds. Our work provides a highly specific gene-activation CRISPRa technology for target cells and verifies its application for parthenogenesis induction in maize.

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