Engineering Foot-and-Mouth Disease Viruses with Improved Growth Properties for Vaccine Development

文献类型： 外文期刊

第一作者： Zheng, Haixue

作者： Zheng, Haixue;Guo, Jianhong;Jin, Ye;Yang, Fan;He, Jijun;Lv, Lv;Zhang, Kesan;Wu, Qiong;Liu, Xiangtao;Cai, Xuepeng

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Background: No licensed vaccine is currently available against serotype A foot-and-mouth disease (FMD) in China, despite the isolation of A/WH/CHA/09 in 2009, partly because this strain does not replicate well in baby hamster kidney (BHK) cells. Methodology/Principal Findings: A novel plasmid-based reverse genetics system was used to construct a chimeric strain by replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09. The chimeric virus displayed growth kinetics similar to those of O/CHA/99 and was selected for use as a candidate vaccine strain after 12 passages in BHK cells. Cattle were vaccinated with the inactivated vaccine and humoral immune responses were induced in most of the animals on day 7. A challenge infection with A/WH/CHA/09 on day 28 indicated that the group given a 4-mu g dose was fully protected and neither developed viremia nor seroconverted to a 3ABC antigen. Conclusions/Significance: Our data demonstrate that the chimeric virus not only propagates well in BHK cells and has excellent antigenic matching against serotype A FMD, but is also a potential marker vaccine to distinguish infection from vaccination. These results suggest that reverse genetics technology is a useful tool for engineering vaccines for the prevention and control of FMD.

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