Pathogenic characteristics of three genotype II porcine reproductive and respiratory syndrome viruses isolated from China

文献类型： 外文期刊

第一作者： Shang, Youjun

作者： Shang, Youjun;Wang, Guangxiang;Yin, Shuanghui;Tian, Hong;Du, Ping;Wu, Jinyan;Chen, Yan;Yang, Shunli;Jin, Ye;Zhang, Keshan;Lu, Zengjun;Liu, Xiangtao

作者机构：

关键词： Immune response;Pathogenic characteristics;Porcine reproductive and respiratory syndrome virus

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2013 年 10 卷

页码：

收录情况： SCI

摘要： Background: We examined differences in pathogenicity in pigs from China that had been experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV). Methods: We compared pathogenic characteristics of a field isolate (GX-1/2008F), two PRRSV isolates (HN-1/2008, YN-1/2008) propagated in cells, and GX-1/2008F that had been propagated in cells (GX-1/2008). The clinical courses, along with humoral and cell-mediated responses, were monitored for 21 days post-infection (DPI). Animals were sacrificed and tissue samples used for gross pathological, histopathological and ultrastructure examination. Results: At 2-3 DPI, animals infected with cell-propagated viruses exhibited signs of coughing, anorexia and fever. However their rectal temperature did not exceed 40.5 degrees C. Viremia was detectable as early as 3 DPI in animals infected with HN-1/2008 and YN-1/2008. Animals inoculated with GX-1/2008F displayed clinical signs at 6 DPI; the rectal temperature of two animals in this group exceeded 41.0 degrees C, with viremia first detected at 7 DPI. Seroconversion for all challenged pigs, except those infected with GX-1/2008, was seen as early as 7 DPI. All of these pigs had fully seroconverted by 11 DPI. All animals challenged with GX-1/2008 remained seronegative until the end of the experiment. Innate immunity was inhibited, with levels of IFN-alpha and IL-1 not significantly different between control and infected animals. The cytokines IFN-gamma and IL-6 transiently increased during acute infection. All virus strains caused gross lesions including multifocal interstitial pneumonia and hyperplasia of lymph nodes. Inflammation of the stomach and small intestine was also observed. Lesions in the group infected with GX-1/2008F were more serious than in other groups. Transmission electron microscopy revealed that alveolar macrophages, plasmacytes and lymphocytes had fractured cytomembranes, and hepatocytes had disrupted organelles and swollen mitochondria. Conclusions: The pathogenicity of the PRRSV field isolate became attenuated when propagated in MARC-145 cells. Tissue tropism of highly pathogenic strains prevailing in China was altered compared with classical PRRSV strains. The observed damage to immune cells and modulation of cytokine production could be mechanisms that PRRSV employs to evade host immune responses.

分类号：

  • 相关文献

[1]LiCl inhibits PRRSV infection by enhancing Wnt/beta-catenin pathway and suppressing inflammatory responses. Hao, Hong-ping,Li, Jia-rong,Wang, Yue,Ni, Bo,Wang, Rui,Wang, Xin,Sun, Ming-xia,Fan, Hong-jie,Mao, Xiang,Wen, Li-bin,Fan, Hong-jie. 2015

[2]Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china. Wang, Lin-jian,Guo, Zhenhua,Qiao, Songlin,Li, Rui,Xie, Sha,Chen, Xin-xin,Zhang, Gaiping,Wang, Lin-jian,Zhang, Gaiping,Wan, Bo,Zhang, Gaiping. 2018

[3]Mechanisms of suppression of interferon production by porcine reproductive and respiratory syndrome virus. Zhu, L.,Zhou, Y.,Tong, G.. 2012

[4]Porcine 2 ', 5 '-oligoadenylate synthetase 2 inhibits porcine reproductive and respiratory syndrome virus replication in vitro. Zhao, Mengmeng,Wan, Bo,He, Jian,Zhang, Gaiping,Li, Huawei,Chen, Xinxin,Wang, Linjian,Wang, Yinbiao,Xie, Sha,Qiao, Songlin,Zhang, Gaiping,Zhang, Gaiping. 2017

[5]Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV. Chen, Changmu,Cui, Shangjin,Zhang, Chaofan,Li, Jun,Wang, Jinbao. 2010

[6]A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. Kang, Kang,Luo, Jun,Kang, Kang,Zhong, Jiasheng,Zhang, Limin,Gou, Deming,Yang, Keli,Tian, Yongxiang,Zhai, Jianxin,Zhang, Li,Song, Changxu,Gou, Christine Yuan. 2014

[7]Development and validation of an immunogold chromatographic test for on-farm detection of PRRSV. Zhou, Sheng-hua,Cui, Shang-jin,Chen, Chang-mu,Zhang, Fan-chao,Li, Jun,Zhou, Shun.

[8]Antiviral activity of phage display selected peptides against Porcine reproductive and respiratory syndrome virus in vitro. Liu, Ke,Feng, Xiuli,Zhou, Bin,Cao, Ruibing,Huang, Li,Pang, Ran,He, Danni,Lian, Xue,Chen, Puyan,Ma, Zhiyong,Luo, Chao,Miao, Denian.

[9]Proteomic Analysis of the Secretome of Porcine Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus. Li, Yuming,Wu, Zhuanchang,Liu, Ke,Qi, Pengfei,Xu, Jinpeng,Wei, Jianchao,Li, Beibei,Shao, Donghua,Shi, Yuanyuan,Qiu, Yafeng,Ma, Zhiyong.

[10]Nonstructural Protein 4 of Porcine Reproductive and Respiratory Syndrome Virus Modulates Cell Surface Swine Leukocyte Antigen Class I Expression by Downregulating beta 2-Microglobulin Transcription. Qi, Pengfei,Liu, Ke,Wei, Jianchao,Li, Yuming,Li, Beibei,Shao, Donghua,Wu, Zhuanchang,Shi, Yuanyuan,Tong, Guangzhi,Qiu, Yafeng,Ma, Zhiyong.

[11]Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology. Wang, Heng,Sun, Lingshuang,Ning, Zhangyong,Zhang, Guihong,Wang, Heng,Sun, Lingshuang,Ning, Zhangyong,Ji, Fangxiao,Cui, Jin,Zhang, Guihong,Liu, Rongchang,Zhang, Weidong.

[12]An infectious clone of the highly pathogenic porcine reproductive and respiratory syndrome virus: Topology of glycoprotein 3 (GP3) addressing the intrachain disulfide bonds. Feng ChunYan,Yan JingHua,Gao, George F.,Feng ChunYan,Gao, George F.,Liu YueHuan,Gao, George F.. 2011

[13]Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China. Wang, Feng-Xue,Guo, Li,Yang, Yan-Ling,Song, Ni,Chen, Li-Zhi,Cheng, Shi-Peng,Wen, Yong-Jun. 2013

[14]Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: A crucial protein in viral pathogenesis, immunity and diagnosis. Wang, Feng-Xue,Song, Ni,Chen, Li-Zhi,Cheng, Shi-Peng,Wen, Yong-Jun,Wu, Hua. 2013

[15]Generation and Immunogenicity of a Recombinant Adenovirus Co-Expressing the E2 Protein of Classical Swine Fever Virus and the GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus. Li Hong-yu,Sun Yuan,Zhang Xing-juan,Chang Tian-ming,Wang Xiang-peng,He Fan,Huang Jun-hua,Qiu Hua-ji. 2011

[16]Proteomic alteration of Marc-145 cells and PAMs after infection by porcine reproductive and respiratory syndrome virus. Ding, Zhuang,Zhang, Xiao-dong,Li, Yang,Li, Zhi-jie,Liu, Chang-jun,Zhang, Yan-Ping,Li, Ya-gang.

[17]Novel Nsp2 deletion based on molecular epidemiology and evolution of porcine reproductive and respiratory syndrome virus in Shandong Province from 2013 to 2014. Wang, Feng-Xue,Liu, Ying,Liu, Xing,Sun, Na,Yang, Yong,Zhu, Hong-Wei,Ren, Jing-Qiang,Cheng, Shi-Peng,Wen, Yong-Jun,Qin, Li-Ting,Chen, Ting,Sun, Ying-Jun.

[18]A conjugate protein containing HIV TAT, ISG20, and a PRRSV polymerase binding inhibits PRRSV replication and may be a novel therapeutic platform. Liu, Ke,Li, Yuming,Huan, Beili,Shao, Donghua,Wei, Jianchao,Qiu, Yafeng,Li, Beibei,Tong, Guangzhi,Ma, Zhiyong,Zhou, Bin,Wang, Feifei,Qian, Yingjuan,Jung, Yong-Sam,Miao, Denian.

[19]Identification of CD8(+) cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice. Zhang, Weijun,Lin, Yan,Bai, Yu,Tong, Tiegang,Wang, Qun,Liu, Nihong,Liu, Guangliang,Xiao, Yihong,Yang, Tao,Bu, Zhigao,Wu, Donglai,Tong, Guangzhi,Zhang, Weijun,Lin, Yan,Bai, Yu,Tong, Tiegang,Wang, Qun,Liu, Nihong,Liu, Guangliang,Xiao, Yihong,Yang, Tao,Bu, Zhigao,Tong, Guangzhi,Wu, Donglai. 2011

[20]Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene. Cai Xue-hui,Han Wen-yu,Cai Xue-hui,Wu Guo-jun,Liu Yong-gang,Shi Wen-da,Wang Shu-jie,Ma Ping,Li Cheng-jun,Liu Guang-qing. 2012

作者其他论文 更多>>

微信小程序