Construction and Characterization of a Bacterial Artificial Chromosome Library from the Huoyan Goose
文献类型： 外文期刊
第一作者： Hu, Peng Fei
作者： Hu, Peng Fei;Li, Xiang Chen;Chen, Xian Wei;Guan, Wei Jun;Ma, Yue Hui
作者机构：
期刊名称：LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION （ 影响因子：0.165； ）
ISSN： 1097-8135
年卷期： 2013 年 10 卷 1 期
页码：
收录情况： SCI
摘要： Huoyan goose is a famous Chinese local breed for its egg productivity. In this study, a bacterial artificial chromosome library of the Huoyan goose was successfully established using the Hind III site of the vector pBeloBAC11, comprising of 115, 200 clones arrayed in 543, 384 well microplates, with an average insert size of 102 kb and the content of the library was 11.4 genome equivalents, which yielded a theoretical probability of 99.93% for isolating a particular DNA sequence. BAC clones of the library were stable in the bacterial host for at least 100 passages.
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