Construction and Characterization of a cDNA Expression Library from the Endangered Jinnan Cattle

文献类型： 外文期刊

第一作者： Hu, Peng Fei

作者： Hu, Peng Fei;Li, Xiang Chen;Wang, Zhi Yu;Guan, Wei Jun;Ma, Yue Hui

作者机构：

关键词： Jinnan cattle;cDNA expression library;endangered animals

期刊名称：LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION （ 影响因子：0.165； ）

ISSN： 1097-8135

年卷期： 2012 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Jinnan cattle is one of the most important species in China, It is also listed as one of the 78 nationally protected domestic animals by the Chinese government in the year of 2000. The construction of cDNA expression library of Jinnan cattle is of great significance for its protection of genetic resources, and it is very important for the research of gene function. In this study, the total RNA was extracted from the ear tissue of Jinnan cattle, then the ear tissue cDNA expression library of Jinnan cattle was constructed using SMART (TM) technique. The result showed that the titer of amplified cDNA library is 1.17x10(10)pfu.mL(-1), the rate of recombinant is above 93.47%, and the average size of the fragments is 0.7 kb. This study has an important significance for the preservation of Jinnan cattle gene resources. [Hu PF, Li XC, Wang ZY, Guan WJ, Ma YH. Construction and Characterization of a cDNA Expression Library from the Endangered Jinnan Cattle. Life Sci J 2012;9(4):3449-3451] (ISSN: 1097-8135). http://www.lifesciencesite.com. 511

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