Measurement of N-15 and O-18 isotope abundance of nitrate using denitrifier method on Tracegas-Isotope Ratio Mass Spectrometry

文献类型: 外文期刊

第一作者: Xu, Chunying

作者: Xu, Chunying;Li, Yuzhong;Li, Qiaozhen;Dong, Yiwei;Fang, Fuli;Guo, Zhicheng

作者机构:

关键词: Denitrifier bacterial;Tracegas-Isotope Ratio Mass Spectrometry;Nitrate;delta N-15 and delta O-18

期刊名称:ADVANCES IN ENVIRONMENTAL TECHNOLOGIES, PTS 1-6

ISSN: 1022-6680

年卷期: 2013 年 726-731 卷

页码:

收录情况: SCI

摘要: A modified denitrifier method on Tracegas-isotope ratio mass spectrometry (IRMS) was developed to determine the nitrogen and oxygen isotopic compositions of nitrate (NO3-) in water samples. The protocol presented in our previous publication was modified in several aspects. Then, the incubation time, amount of NO3- sample, and effect of trapping time in the Tracegas system were analyzed using the standard reference USGS34. Both delta N-15 and delta O-18 values demonstrated accurate and reproducible at incubation times exceeding 12 h or overnight as well as added 0.2 mu g to 0.4 mu g NO3--N samples. Our data also showed that 100 s for trapping time in Tracegas system was adequate to achieve accurate and reproducible results. Finally, the delta N-15 and delta O-18 values of nitrate in animal wastewater were measured using this method. The results indicate that the denitrifier method can be used widely for tracing nitrate sources and controlling nitrate contamination in China.

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