Production of Bacteriocin E50-52 by Small Ubiquitin-Related Modifier Fusion in Escherichia coli

文献类型： 外文期刊

第一作者： Wang, Qing

作者： Wang, Qing;Fu, Wenjuan;Ma, Qingshan;Yu, Zhanqiao;Zhang, Rijun

作者机构：

关键词： Escherichia coli;bacteriocin E50-52;purification;recombinant expression;Small ubiquitin-related modifier

期刊名称：POLISH JOURNAL OF MICROBIOLOGY （ 影响因子：1.28； 五年影响因子：1.352 ）

ISSN： 1733-1331

年卷期： 2013 年 62 卷 4 期

页码：

收录情况： SCI

摘要： Bacteriocin E50-52, a class Ha bacteriocin with a wide antibacterial spectrum, and has a huge potential to be a substitute for conventional antibiotics. In this research, the bacteriocin E50-52 gene was cloned into the expression vector pET SUMO (small ubiquitin-related modifier) and introduced into Escherichia coli BL21 (DE3). The recombinant fusion protein SUMO-bacteriocin E50-52 expressed in a soluble form was purified to a purity of more than 90% by Ni-NTA sepharose column and 117 mg fusion protein was obtained per liter of fermentation culture. The fusion protein was cleaved with SUMO protease and re-applied to a Ni-NTA Sepharose column. Finally, about 16 mg recombinant bacteriocin E50-52 (rbE50-52) was obtained from a 1-liter fermentation culture with no less than 95% purity. The rbE50-52 had similar antimicrobial properties and molecular weight as the native bacteriocin E50-52 and showed very low hemolytic activity.

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