Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development

文献类型： 外文期刊

第一作者： Zhang, Ye

作者： Zhang, Ye;Sun, Jinbo;Xia, Han;Zhao, Chuanzhi;Hou, Lei;Li, Aiqin;Zhao, Shuzhen;Wang, Xingjun;Zhang, Ye;Sun, Jinbo;Xia, Han;Zhao, Chuanzhi;Hou, Lei;Li, Aiqin;Zhao, Shuzhen;Wang, Xingjun;Sun, Jinbo;Xia, Han;Zhao, Chuanzhi;Wang, Baoshan;Chen, Min;Zhao, Shuzhen;Wang, Xingjun

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Arachis hypogaea L. geocarpy is a unique feature different from other legume plants. Flowering and fertilization occur above ground, while the following processes of pod formation and development proceed in the soil. The zygote divides only few times to develop into pre embryo and then further embryo developmental process stops when the gynoecium is exposed to light condition or normal day/night period. In this study, eight phytochrome genes were identified in two wild peanuts (four in Arachis duranensis and four in Arachis ipaensis). Using RACE and homologous cloning, the full CDS of AhphyA, AhphyA-like, AhphyB and AhphyE were acquired in cultivated peanut. Protein structure analysis showed that the conservative coding domains of phytochromes from a number of other plant species were found in these proteins. The C-terminal of AhphyA, AhphyA-like and AhphyB could interact with phytochrome-interacting factor 3 in vitro. The expression patterns of these genes in various tissues were analyzed by qRT-PCR, and significant differences were observed. Interestingly, the expression levels of AhphyA-like changed significantly during gynophore growth and early pod development. Furthermore, protein accumulation patterns of AhphyA and AhphyB in gynophore were different during early pod development stages in that AhphyA and AhphyB proteins were not detected in Si and S2 gynophores, while significant accumulation of AhphyA and AhphyB were detected in S3 gynophore. These results provided evidence that phytochromes mediated light signal transduction may play key roles in peanut geocarpy development.

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