农科机构知识库联盟

Development of Polymorphic Simple Sequence Repeat Markers in Lilium regale by Magnesphere Method

文献类型：外文期刊

第一作者： Zhao, Y. P.

作者： Zhao, Y. P.;Wang, W. H.;Zhao, X. Y.;Wang, S. D.;Liu, G. S.;Qin, T. F.

作者机构：

关键词： L. regale;magnesphere method;library screening;SSR markers

期刊名称：INTERNATIONAL CONFERENCE ON GERMPLASM OF ORNAMENTALS

ISSN： 0567-7572

年卷期： 2013 年 977 卷

页码：

收录情况： SCI

摘要： Simple sequence repeat (SSR) is one of the most useful molecular markers based on DNA length polymorphism and an efficient tool for population genetic studies and primary genetic linkage maps construction. In this study, we selected lily 'Lilium regale' as a model material. Genomic DNA was extracted and digested with restriction enzyme Mse1, and ligated with adapters. After PCR, the fragments were hybridized with biotin-labeled simple sequence repeat probes (AG)(15) and (GT)(15), the nonspecific adsorptions were washed away by using 0.1xSSR, then the enriched product of (AG)(n) and (GT)(n) were obtained. The SSR containing sequences were amplified using primers designed complementary to linkers, cloned into pMD18-T vectors and transformed into E. coli, two SSR-enriched libraries (AG and GT) were constructed. Screening positive clones by PCR cloning method, 192 and 48 clones were screened in AG-enriched and GT-enriched library respectively. 109 and 9 SSR-containing sequences were obtained, the concentration rates were 56.8 and 16.7%. The result showed the concentration rate of AG-enriched library is higher than GT-enriched library. After sequence analysis, 36 pairs of SSR primers were designed successfully, then the primers were used to amplify the genomic DNA of 15 wide lilies genotypes with diversified origin, 18 SSR primers had amplified polymorphism, but only 2 primers had amplification in all the 15 lilies, cross of SSR primers among lily species was low, suggesting the genetic relationship between wild species of lily was far, more in-depth research is needed in the future.

分类号：

相关文献

[1]Fosmid library construction and screening for the maize mutant gene Vestigial glume 1. Liu, Chaoxian,Liu, Xiaoli,Cai, Yilin,Lei, Lei,Guan, Haiying. 2016

[2]QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli. Asad Muhammad Azeem,BAI Bin,LAN Cai-xia,YAN Jun,XIA Xian-chun,ZHANG Yong,HE Zhong-hu. 2013

[3]Identification of stable quantitative trait loci (QTLs) for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population. Muhammad Jamshed,Fei Jia,Juwu Gong,;Koffi Kibalou Palanga,Yuzhen Shi,Junwen Li,Haihong Shang,Aiying Liu,Tingting Chen,Zhen Zhang,Juan Cai,Qun Ge,Zhi Liu,Quanwei Lu,Xiaoying Deng,Yunna Tan,Harun or Rashid,Zareen Sarfraz,Murtaza Hassan,Wankui Gong,Youlu Yuan. 2016

[4]Availability of Two-dimensional Bulked DNA Sampling in Uniformity Assessment of 8 Inbred Rice Lines. Liu Yan-Fang,Huang Qing-Mei,Yang Xiao-Hong,Li Yan-Gang,Zhang Peng,Wang Jiang -Min,Zhang Hui,Guan Jun-Jiao,Zhang Jian-Hua. 2016

[5]Fingerprinting 128 Chinese clonal tea cultivars using SSR markers provides new insights into their pedigree relationships. Tan, Li-Qiang,Peng, Min,Xu, Li-Yi,Chen, Sheng-Xiang,Zou, Yao,Qi, Gui-Nian,Wang, Li-Yuan,Cheng, Hao,Wang, Li-Yuan. 2015

[6]Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Chen, XM,Luo, YH,Xia, XC,Xia, LQ,Chen, X,Ren, ZL,He, ZH,Jia, JZ. 2005

[7]Linkage and mapping analyses of the densonucleosis non-susceptible gene nsd-Z in the silkworm Bombyx mori using SSR markers. Li, MW,Guo, QH,Hou, CX,Miao, XX,Xu, AY,Guo, XJ,Huang, YP. 2006

[8]SSR markers associated with fertility restoration genes against Triticum timopheevii cytoplasm in Diticum aestivum. Zhou, WC,Kolb, FL,Domier, LL,Wang, SW. 2005

[9]Establishment of Molecular Identity Cards for Cucumis melo Cultivars Using SSR Markers. Li, Li,Xu, Xiulan,Wu, Ping,Li, Li,Xu, Xiulan,Wu, Ping,Li, Li,Xu, Xiulan,Wu, Ping,Zhang, Guo,Zhang, Xiaobing. 2018

[10]Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai. Fu, Bisheng,Zhang, Zhiliang,Zhang, Qiaofeng,Wu, Xiaoyou,Wu, Jizhong,Cai, Shibin,Zhang, Zhiliang. 2017

[11]Evaluation of the genetic diversity of Asian peach accessions using a selected set of SSR markers. Xie, Rangjin,Li, Xiongwei,Chai, Mingliang,Song, Lijuan,Jia, Huijuan,Gao, Zhongshan,Wu, Dajun,Chen, Miaojin,Chen, Keming,Jose Aranzana, Maria,Xie, Rangjin.

[12]Inheritance and QTL mapping of resistance to gummy stem blight in cucumber stem. Zhang, Shengping,Liu, Shulin,Miao, Han,Shi, Yanxia,Wang, Min,Wang, Ye,Li, Baoju,Gu, Xingfang.

[13]Development and characterization of SSR markers in lychee (Litchi chinensis). Li, Mingfang,Zheng, Xueqing,Zhu, Yongqiang,Wang, Xiangshe,Liang, Suyu,Li, Lei,Wu, Xingrong. 2006

[14]Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing. Fang, D. -A.,Xu, P.,Fang, D. -A.,Zhou, Y. -F.,Duan, J. -R.,Zhang, M. -Y.,Xu, D. -P.,Liu, K.,Xu, P.,Wei, Q.. 2015

[15]Association between Chloroplast and Mitochondrial DNA sequences in Chinese Prunus genotypes (Prunus persica, Prunus domestica, and Prunus avium). Pervaiz, Tariq,Sun, Xin,Zhang, Yanyi,Tao, Ran,Fang, Jinggui,Zhang, Junhuan. 2015

[16]Genome-wide assessment of genetic diversity and fiber quality traits characterization in &ITGossypium hirsutum&IT races. Kiflom Weldu Okubazghi,Liu Fang,Wang Kun-bo,LI Xiao-na,CAI Xiao-yan,WANG Xing-xing,CHEN Hao-dong,HOU Zhong-li,WANG Chun-ying,WANG Yu-hong,LIU Fang,WANG Kun-bo. 2017

[17]Molecular mapping of quantitative trait loci for adult-plant resistance to powdery mildew in Italian wheat cultivar Libellula. M. A. Asad,B. Bai,C. X. Lan,J. Yan,X. C. Xia,Y. Zhang,Z. H. He. 2012

[18]Transcriptome analysis of salt-responsive genes and SSR marker exploration in Carex rigescens using RNA-seq. Li Ming-na,Feng Zi-rong,Sun Yan,Zhang, Kun,Cao Shi-hao,Long Rui-cai,Kang Jun-mei,Wang Zhen,Liu Feng-qi. 2018

[19]Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China. Chen, Z. -W.,Lu, R. -J.,Zou, L.,Du, Z. -Z.,Gao, R. -H.,He, T.,Huang, J. -H.,Chen, Z. -W.,Lu, R. -J.,Zou, L.,Du, Z. -Z.,Gao, R. -H.,He, T.,Huang, J. -H.. 2012

[20]Assessment of wheat variety distinctness using SSR markers. Wang Li-xin,Liu Li-hua,Li Hong-bo,Pang Bin-shuang,Zhao Chang-ping,Qiu Jun,Chang Li-fang. 2015

作者其他论文更多>>

miRNA expression analysis of the sheep follicle during the prerecruitment, dominant, and mature stages of development under FSH stimulation

作者：Yuan, H.;Lu, J.;Xiao, S. Y.;Han, X. Y.;Song, X. T.;Yang, C. X.;Yao, Y. C.;Yuan, H.;Lu, J.;Xiao, S. Y.;Han, X. Y.;Song, X. T.;Yang, C. X.;Yao, Y. C.;Qi, M. Y.;Liu, G. S.

关键词：Follicular development; Ovarian steroidogenesis; FSH stimulation; miRNAs; Oxidative stress
Transcriptome analysis of sheep follicular development during prerecruitment, dominant, and mature stages after FSH superstimulation

作者：Yao, Y. C.;Song, X. T.;Zhai, Y. F.;Liu, S.;Lu, J.;Xu, X.;Zhang, J. N.;Huang, H.;Yuan, H.;Yao, Y. C.;Song, X. T.;Zhai, Y. F.;Liu, S.;Lu, J.;Xu, X.;Zhang, J. N.;Huang, H.;Yuan, H.;Qi, M. Y.;Liu, Y. F.;Liu, G. S.

关键词：RNA-seq; Ovarian steroidogenesis; Sheep; Follicular development; FSH superstimulation
SELECTING THE SUPERIOR GENOTYPE OF SUMMER MAIZE HYBRIDS IN MEGA-ENVIRONMENTS USING AMMI MODEL AND GGE BIPLOT IN CHINA

作者：Wang, S. Q.;Wang, S. D.;Guo, Q.;Chen, Z. Y.

关键词：genotype by environment interaction; stability; adaptability; agronomic traits; yield; agronomic traits
Over-expression of transcription factor GhWRI1 in upland cotton

作者：Liu, Z. J.;Zhao, Y. P.;Liang, W.;Cui, Y. P.;Hua, J. P.;Wang, Y. M.

关键词：Gossypium hirsutum; transcription factor; transgenic plants
Cloning and expression analysis of terpenoid metabolism-related gene NsyCMS in Nicotiana sylvestris

作者：Lv, J.;Liu, G. S.;Li, W.;Hussian, Z.;Sun, Y. H.

关键词：Nicotiana sylvestris;Fluorogenic quantitative PCR;2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;Terpene biosynthesis
Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

作者：Huang, X. Y.;Tao, P.;Li, B. Y.;Wang, W. H.;Yue, Z. C.;Lei, J. L.;Zhong, X. M.;Huang, X. Y.

关键词：Chinese cabbage;Gene expression;Hsf;Interaction network;Phylogenetic analysis
Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco

作者：Ding, A. M.;Li, F. X.;Chen, Y. Q.;Zong, P.;Qu, X.;Gong, D. P.;Liu, G. S.;Sun, Y. H.;Ding, A. M.;Chen, Y. Q.;Zong, P.;Qu, X.

关键词：Rf gene;Pentatricopeptide repeat protein family;Quantitative reverse transcription polymerase chain reaction;Sequence structure analysis;Phylogenetic tree

Development of Polymorphic Simple Sequence Repeat Markers in Lilium regale by Magnesphere Method

作者其他论文 更多>>

miRNA expression analysis of the sheep follicle during the prerecruitment, dominant, and mature stages of development under FSH stimulation

Transcriptome analysis of sheep follicular development during prerecruitment, dominant, and mature stages after FSH superstimulation

SELECTING THE SUPERIOR GENOTYPE OF SUMMER MAIZE HYBRIDS IN MEGA-ENVIRONMENTS USING AMMI MODEL AND GGE BIPLOT IN CHINA

Over-expression of transcription factor GhWRI1 in upland cotton

Cloning and expression analysis of terpenoid metabolism-related gene NsyCMS in Nicotiana sylvestris

Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco

作者其他论文更多>>