The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

文献类型: 外文期刊

第一作者: Geng Li-li

作者: Geng Li-li;Shu Chang-long;Song Fu-ping;Zhang Jie;Jiang Wei-yu;Lin Yi;Dai Ping-li;Zhou Ting;Lang Zhi-hong

作者机构:

关键词: Bacillus thuringiensis;intestinal bacteria;horizontal gene transfer;transgenic maize;Apis cerana;biosafety

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2013 年 12 卷 3 期

页码:

收录情况: SCI

摘要: Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and Conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear cry1Ah gene (800 ng mL(-1)) and supercoiled plasmid DNA (800 ng mL(-1)) under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon's index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic cry1Ah maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.

分类号:

  • 相关文献

[1]Effect of Bt broccoli and resistant genotype of Plutella xylostella (Lepidoptera: Plutellidae) on life history and prey acceptance of the predator Coleomegilla maculata (Coleoptera: Coccinellidae). Liu, Xiaoxia,Han, Fei,Zhang, Qingwen,Liu, Xiaoxia,Abro, Ghulam Hussain,Han, Fei,Tian, Junce,Chen, Mao,Shelton, Anthony M.,Abro, Ghulam Hussain,Han, Fei,Tian, Junce,Onstad, David,Roush, Rick.

[2]Early instar response to plant-delivered Bt-toxin in a herbivore (Spodoptera litura) and a predator (Propylaea japonica). Zhang, GF,Wan, FH,Liu, WX,Guo, HY. 2006

[3]Effects of brewer's yeast hydrolysate on the growth performance and the intestinal bacterial diversity of largemouth bass (Micropterus salmoides). Zhou, Meng,Liang, Risheng,Mo, Jinfeng,Yang, Sen,Gu, Na,Wu, Zaohe,Babu, Sarath, V,Huang, Yunmao,Lin, Li,Li, Jun,Lin, Li,Li, Jun,Lin, Li. 2018

[4]Effects of the antibiotic growth promoters flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia (Oreochromis niloticus female x O. aureus male). He, Suxu,Zhou, Zhigang,Liu, Yuchun,Cao, Yanan,Meng, Kun,Shi, Pengjun,Yao, Bin,Ringo, Einar. 2010

[5]The optimization of fermentation conditions and enzyme properties of Stenotrophomonas maltophilia for protease production. Wang, Zaigui,Sun, Linghong,Cheng, Jia,Liu, Chaoliang,Liu, Ying,Tang, Xiangfang,Zhang, Hongfu.

[6]Effects of partially replacing dietary soybean meal or cottonseed meal with completely hydrolyzed feather meal (defatted rice bran as the carrier) on production, cytokines, adhesive gut bacteria, and disease resistance in hybrid tilapia (Oreochromis niloticus female x Oreochromis aureus male). Zhang, Zhen,Xu, Li,Liu, Wenshu,Yang, Yalin,Zhou, Zhigang,Du, Zhenyu.

[7]Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains. Liu, Wenshu,Gu, Zemao,Liu, Wenshu,Ren, Pengfei,He, Suxu,Xu, Li,Yang, Yaling,Zhou, Zhigang.

[8]Complementary DNA (cDNA) cloning and functional verification of resistance to head smut disease (Sphacelotheca reiliana) of an NBS-LRR gene ZmNL in maize (Zea mays). Di, Hong,Deng, Yanxue,Dong, Xiaojie,Li, Rou,Zhou, Yu,Wang, Zhenhua,Yu, Tao.

[9]The intergenic region of the maize defensin-like protein genes Def1 and Def2 functions as an embryo-specific asymmetric bidirectional promoter. Liu, Xiaoqing,Yang, Wenzhu,Li, Ye,Li, Suzhen,Zhou, Xiaojin,Zhao, Qianqian,Fan, Yunliu,Lin, Min,Chen, Rumei,Li, Suzhen.

[10]Assessment of transgene copy number and zygosity of transgenic maize overexpressing Cry1Ie gene with SYBRA (R) Green qRT-PCR. Zhang, Yuwen,Wang, Guoying,Liu, Yunjun,Zhang, Yuwen,Liu, Yan,Zhang, Jie,Wang, Jianhua.

[11]Developing transgenic maize (Zea mays L.) with insect resistance and glyphosate tolerance by fusion gene transformation. Lang Zhi-hong,Lu Wei,Zhu Li,Lin Min,Huang Da-fang,Zhang Jie,He Kang-lai. 2015

[12]Overexpression of a modified AM79 aroA gene in transgenic maize confers high tolerance to glyphosate. Ren Zhen-jing,Cao Gao-yi,Zhang Yu-wen,Liu Yan,Liu Yun-jun,Cao Gao-yi,Zhang Yu-wen,Liu Yan. 2015

[13]Efficacy Evaluation of Two Transgenic Maize Events Expressing Fused Proteins to Cry1Ab-Susceptible and -Resistant Ostrinia furnacalis (Lepidoptera: Crambidae). Liu, G. G.,Shen, Z. C.,Ye, G. Y.,He, K. L.,Peng, Y. F.. 2013

[14]The influence of Bt-transgenic maize pollen on the bacterial diversity in the midgut of Apis mellifera ligustica. Cui, Hong-Juan,Shu, Chang-Long,Song, Fu-Ping,Zhang, Jie,Dai, Ping-Li,Zhou, Ting,Lang, Zhi-Hong. 2013

[15]Field observations of Ostrinia nubilalis eclosion and post-eclosion activity of females around their natal plants. Hu, Yang,Hu, Yang,Andow, David A.. 2011

[16]Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize. Zhang, Yuwen,Ren, Yuan,Wang, Jianhua,Zhang, Yuwen,Liu, Yunjun,Liu, Yan,Wang, Guoying,Liang, Gemei,Song, Fuping,Bai, Shuxiong. 2013

[17]A 90-day toxicity study of GmTMT transgenic maize in Sprague-Dawley rats. Fang, Jin,Feng, Yongquan,Zhi, Yuan,Yu, Zhou,Jia, Xudong,Zhang, Lan.

[18]A scientific note on detection of honeybee viruses in the darkling beetle (Alphitobius diaperinus, Coleoptera: Tenebrionidae), a new pest in Apis cerana cerana colonies. Li, Zhiguo,Huang, Shaokang,Huang, Wei-fone,Geng, Haiyang,Li, Meng,Su, Songkun,Zhao, Yazhou,Chen, Yanping.

[19]Managed honeybee colony losses of the Eastern honeybee (Apis cerana) in China (2011-2014). Chen, Chao,Liu, Zhiguang,Chen, Xiao,Guo, Haikun,Wang, Huihua,Tang, Jiao,Shi, Wei,Chen, Chao,Liu, Zhiguang,Shi, Wei,Luo, Yuexiong,Xu, Zheng,Wang, Shunhai,Zhang, Xuewen,Dai, Rongguo,Gao, Jinglin.

[20]Nosema ceranae n sp (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis cerana (Hymenoptera, Apidae). Fries, I,Feng, F,daSilva, A,Slemenda, SB,Pieniazek, NJ. 1996

作者其他论文 更多>>