Detection of Mink (Mustela vison) DNA in Meat Products using Polymerase Chitin Reaction PCR Assay

文献类型： 外文期刊

第一作者： Sun, Weili

作者： Sun, Weili;Li, Guangyu;Liu, Hanlu;Zhong, Wei;Zhang, Haihua;Bao, Kun;Xu, Chao;Yang, Yahan;Wang, Zhuo;Sun, Weili;Li, Guangyu;Liu, Hanlu;Zhong, Wei;Zhang, Haihua;Bao, Kun;Xu, Chao;Wang, Zhuo;Yang, Yahan

作者机构：

关键词： DNA fragments;meat products;mink;polymerase chain reaction

期刊名称：ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.869； ）

ISSN： 1683-9919

年卷期： 2012 年 7 卷 12 期

页码：

收录情况： SCI

摘要： The study was to develop a Polymerase Chain Reaction (PCR) assay for specific detection of mink meat using designed pairs of primers based on mitochondrial D-loop. Mink meat is used as fraud ingredients of false mutton or dog-meat in meat markets. This study was conducted to establish Polymerase Chain Reaction (PCR) method for the sensitive and specific detection of mink (Mustela vison) DNA in meat products. Six pairs of primers were designed from. tandem repeat region of D-loop in mitochondria after alignment of the available sequences in the GenBank database. The specific pair of primers chosen from the six designed pairs by PCR generated specific fragments of 343 bp in length for mink. The specificity of detection was conducted with DNA samples of mink, blue fox, dog, raccoon dog, swine, sheep. Then amplification of positive reaction was observed only in mink species. In this study, no adverse effects of cooking and autoclaving were found on amplification of mink DNA fragments. Then the detection limit was found to be less than 1% in mixed meat products. The PCR method described in this study proved to be very sensitive and reliable in mink DNA identification. Thus, it could be considered as a further improvement method for the detection of mink DNA in meat products processed under different manufacturing conditions.

分类号：

  • 相关文献

[1]Determination of Inosine Monophosphate in Meat Products by Ion Chromatography with Suppressed Conductivity Detection. Zhu Zuo-Yi,Zhang Yu,Wang Jun-Hong,Li Xue,Wang Wei,Zhu Zuo-Yi,Zhang Yu,Wang Wei,Zhu Zuo-Yi,Zhang Yu,Wang Wei. 2015

[2]Acetonitrile extraction coupled with UHPLC-MS/MS for the accurate quantification of 17 heterocyclic aromatic amines in meat products. Yan, Yan,You, Feng-hui,Zhang, Shuang,Tao, Guan-jun,Chen, Jie,Zeng, Mao-mao,Zeng, Mao-mao. 2017

[3]Applicability of accelerated solvent extraction for synthetic colorants analysis in meat products with ultrahigh performance liquid chromatography-photodiode array detection. Liao, Qie Gen,Li, Wei Hong,Luo, Lin Guang.

[4]Application of formulated diets and their effects on nutrient digestibility and reproductive performance of female mink (Neovison vison) during gestation. Jiang, Qingkui,Li, Guangyu,Zhang, Tietao,Zhang, Haihua,Xing, Xiumei,Yang, Fuhe,Gao, Xiuhua. 2017

[5]Effects of different dietary protein levels and DL-methionine supplementation on hair growth and pelt quality in mink (Neovision vision). Zhang, H. H.,Jiang, Q. K.,Sun, W. L.,Xu, C.,Cong, B.,Yang, F. H.,Li, G. Y.. 2013

[6]Fecal progestin concentrations as an indicator of reproductive success in American Mink. Cao, Xinyan,Wei, Haijun,Xue, Hailong,Li, Xiaoxia,Zhao, Weigang,Xu, Chao,Wang, Shiyong,Diao, Yunfei,Xu, Baozeng,Cao, Xinyan,Wei, Haijun,Xue, Hailong,Li, Xiaoxia,Zhao, Weigang,Xu, Chao,Wang, Shiyong,Diao, Yunfei,Xu, Baozeng,Rose, Jack.

[7]Influence of Dietary Zinc and Copper on Apparent Mineral Retention and Serum Biochemical Indicators in Young Male Mink (Mustela vison). Wu, Xuezhuang,Liu, Zhi,Guo, Jungang,Wan, Chunmeng,Cui, Hu,Gao, Xiuhua,Wu, Xuezhuang,Zhang, Tietao,Yang, Fuhe.

[8]Effects of dietary copper on nutrient digestibility, tissular copper deposition and fur quality of growing-furring mink (Mustela vison). Wu, Xuezhuang,Liu, Zhi,Gao, Xiuhua,Wu, Xuezhuang,Zhang, Tietao,Yang, Fuhe,Wu, Xuezhuang,Zhang, Tietao,Yang, Ying,Yang, Fuhe.

[9]Effects of Different Dietary Manganese Levels on Growth Performance and N Balance of Growing Mink (Neovision vision). Zhang, H. H.,Zhou, N.,Zhang, T. T.,Bao, K.,Xu, C.,Song, X. C.,Zhou, N..

[10]Cloning, expression and antiviral activity of mink alpha-interferons. Zhang, Hai-ling,Zhao, Jian-jun,Chai, Xiu-li,Zhang, Lei,Bai, Xue,Hu, Bo,Liu, Hao,Yan, Xi-jun,Zhang, Dong-liang,Ye, Ming,Wu, Wei. 2015

[11]Detection and Characterization of Avastrovirus Associated with Diarrhea Isolated from Minks in China. Sun, Na,Yang, Yong,Shao, Xi-Qun,Zhang, Shu-Qin,Wang, Feng-Xue,Tan, Bin,Cheng, Shi-Peng,Wen, Yong-Jun,Wang, Gui-Sheng,Tian, Fu-Lin.

[12]Pathogenesis of canine distemper virus in experimentally infected raccoon dogs, foxes, and minks. Zhao, Jianjun,Shi, Ning,Sun, Yangang,Zhu, Chunsheng,Zhang, Hailing,Hu, Bo,Bai, Xue,Yan, Xijun,Zhao, Jianjun,Yan, Xijun,Martella, Vito,Nikolin, Veljko.

[13]Effects of sources and concentrations of zinc on growth performance, nutrient digestibility, and fur quality of growing-furring female mink (Mustela vison). Cui, H.,Nie, H.,Wang, Z. C.,Shi, B.,Gao, X. H.,Cui, H.,Gao, X. H.,Zhang, T. T.,Zhang, X. L.,Yang, F. H.. 2017

[14]Cloning and expression of mink (Neovison vison) interferon-gamma gene and development of an antiviral assay. Zhang, Hailing,Zhao, Jianjun,Bai, Xue,Zhang, Lei,Hu, Bo,Liu, Hao,Xu, Shujuan,Yan, Xijun,Zhang, Hailing,Yan, Xijun,Zhang, Dongliang,Fan, Sining.

[15]Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Zhao, Jian-Jun,Yan, Xi-Jun,Chai, Xiu-Li,Luo, Guo-Liang,Zhang, Hai-Ling,Gao, Han,Bai, Xue,Zhang, Lei,Chen, Tao,Xu, Lei,Zhao, Chun-Fei,Wang, Feng-Xue,Shao, Xi-Qun,Wu, Wei,Cheng, Shi-Peng,Martella, Vito,Liu, Ying-Xue.

[16]Effects of Different Sources and Levels of Copper on Growth Performance, Nutrient Digestibility, and Elemental Balance in Young Female Mink (Mustela vison). Wu, Xuezhuang,Liu, Zhi,Guo, Jungang,Gao, Xiuhua,Wu, Xuezhuang,Zhang, Tietao,Zheng, Junjun,Yang, Fuhe,Wu, Xuezhuang,Zhang, Tietao,Yang, Fuhe.

[17]The identification, typing, and antimicrobial susceptibility of Pseudomonas aeruginosa isolated from mink with hemorrhagic pneumonia. Qi, Jing,Li, Lulu,Du, Yijun,Wang, Jinwen,Luo, Yanbo,Liu, Yuqing,Qi, Jing,Du, Yijun,Wang, Jinwen,Wang, Guisheng,Hu, Ming,Yan, Shigan,Liu, Yuqing,Wang, Shourong,Che, Jie,Lu, Jinxing,Liu, Hui,Hu, Guangchun,Li, Jixia,Gong, Yanwen,Wang, Guisheng.

[18]A simplified genomic DNA extraction protocol for pre-germination genotyping in rice. Duan, Y. B.,Zhao, F. L.,Chen, H. D.,Sheng, W.,Teng, J. T.,Zhang, A. M.,Xue, J. P.,Li, H.,Ni, D. H.,Wei, P. C.. 2015

[19]Molecular characterisation of canine parvovirus strains circulating in China. Hou, S. H.,Zhu, H. F.,Zhang, Q.,Zhai, G. Q.,Wang, Z.,Xu, X. M.. 2010

[20]The complete mitochondrial genome of Paracheirodon axelrodi (Characiformes: Characidae: Paracheirodon). Zhang, Songhao,Sun, Jinsheng,Zhang, Songhao,Cui, Jun,Xu, Ru,Xu, Peng. 2016

作者其他论文 更多>>

微信小程序