Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus

文献类型： 外文期刊

第一作者： Tian, Hong

作者： Tian, Hong;Wu, Jingyan;Chen, Yan;Liu, Xiangtao;Zhang, Keshan;Shang, Youjun

作者机构：

关键词： Sheep pox virus;SYBR Green I based quantitative PCR

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2012 年 9 卷

页码：

收录情况： SCI

摘要： Background: In this study, we developed a SYBR Green-based real-time PCR assay for the detection of sheep pox virus using a plasmid construct carrying one of the highly conserved genes encoding the virion envelope protein (P32) as a template. Results: The method was demonstrated to be highly sensitive, allowing a precise SPV DNA quantitation over a range of nine orders of magnitude (from 101 to 109 copies of standard DNA). Then, specimens from SPV suspected sheep were analyzed by conventional gel-based PCR, real-time PCR and sequence analysis. Conclusion: Comparison between these different techniques revealed that real-time PCR is more sensitive than conventional gel-based PCR, allowing detection low viral titers of SPV in infected sheep.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序