文献类型： 外文期刊
第一作者： Tian, Hong
作者： Tian, Hong;Wu, Jingyan;Chen, Yan;Liu, Xiangtao;Zhang, Keshan;Shang, Youjun
作者机构：
关键词： Sheep pox virus;SYBR Green I based quantitative PCR
期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）
ISSN： 1743-422X
年卷期： 2012 年 9 卷
页码：
收录情况： SCI
摘要： Background: In this study, we developed a SYBR Green-based real-time PCR assay for the detection of sheep pox virus using a plasmid construct carrying one of the highly conserved genes encoding the virion envelope protein (P32) as a template. Results: The method was demonstrated to be highly sensitive, allowing a precise SPV DNA quantitation over a range of nine orders of magnitude (from 101 to 109 copies of standard DNA). Then, specimens from SPV suspected sheep were analyzed by conventional gel-based PCR, real-time PCR and sequence analysis. Conclusion: Comparison between these different techniques revealed that real-time PCR is more sensitive than conventional gel-based PCR, allowing detection low viral titers of SPV in infected sheep.
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