DEVELOPMENT OF 161 NOVEL EST-SSR MARKERS FROM LATHYRUS SATIVUS (FABACEAE)
文献类型: 外文期刊
第一作者: Sun, Xue-Lian
作者: Sun, Xue-Lian;Yang, Tao;Guan, Jian-Ping;Ma, Yu;Jiang, Jun-Ye;Zong, Xu-Xiao;Cao, Rui;Burlyaeva, Marina;Vishnyakova, Margarita;Semenova, Elena;Bulyntsev, Sergey
作者机构:
关键词: expressed sequence tag;Fabaceae;grasspea;Lathyrus sativus;microsatellite;primer
期刊名称:AMERICAN JOURNAL OF BOTANY ( 影响因子:3.844; 五年影响因子:3.657 )
ISSN: 0002-9122
年卷期: 2012 年 99 卷 10 期
页码:
收录情况: SCI
摘要: Premise of the study: Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. Methods and Results: Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. Conclusions: These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea.
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