Establishment of a duck cell line susceptible to duck hepatitis virus type 1

文献类型： 外文期刊

第一作者： Fu, Yuzhi

作者： Fu, Yuzhi;Chen, Zongyan;Li, Chuanfeng;Liu, Guangqing;Fu, Yuzhi

作者机构：

关键词： Duck hepatitis virus (DHV);Embryo fibroblasts cell;DEF-TA cells

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2012 年 184 卷 1-2 期

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收录情况： SCI

摘要： Until recently, there was no cell line that could produce continuously high-titer duck hepatitis virus type 1 (DHV-1). In this study, a duck embryo fibroblast (DEF) cell line was established, and the susceptibility of this cell line to DHV-1 was determined. The primary culture of DEF cells was from a duck embryo that was partially digested with trypsin. Digested tissue pieces were cultured at 37 degrees C in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum. The cultured DEF cells, which had the morphology of fibroblast, proliferated to 100% confluence four days later. An immortalized DEF cell line, named DEF-TA, was established and subcultured to passage 33, and the susceptibility of that cell line to DHV-1 was determined. In the DHV-1 susceptibility tests, cytopathic effects and the propagation of virus were observed in DEF-TA cells after DHV-1 infection. This continuous DHV-1-susceptible DEF cell line may serve as a valuable cell line for studies of cell-virus interactions and the pathogenesis of DHV-1 and may be useful for the development of an inactivated vaccine. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.

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