Identification of porcine serum proteins modified in response to HP-PRRSV HuN4 infection by two-dimensional differential gel electrophoresis

文献类型： 外文期刊

第一作者： An, Tongqing

作者： An, Tongqing;Gong, Daqing;Peng, Jinmei;Leng, Chaoliang;Yuan, Zhonghua;Tian, Zhijun;Yang, Yongqian;Li, Dengyun;Zhang, Deli;Tong, Guangzhi

作者机构：

关键词： DIGE;Serum;HP-PRRSV;Inflammation;Acute phase protein

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2012 年 158 卷 3-4 期

页码：

收录情况： SCI

摘要： Since 2006, highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become the major pathogen attributed to the prevalent porcine reproductive and respiratory syndrome (PRRS) in China. The present study aims to identify serum proteins modified in response to infection of HuN4, a HP-PRRSV strain isolated from a farm in 2006. 2-D DICE analysis allowed for the detection of 19 differentially expressed protein spots, of which 18 were identified by MALDI-TOF/TOF MS. These 18 spots represented for a total of 9 proteins (6 up-regulated and 3 down-regulated), most of which belonged to the acute phase proteins in swine and showed a trend of regression in the late phase of the experiment. One of a series of AGP spots was identified for the first time to be decreased in acute phase of PRRSV infection in swine. But the whole level of the protein in the serum did not show significant changes by Western blot. The rising tendency of Hp was confirmed by Western blot and ELISA. These altered proteins were probably involved in the inflammatory process triggered by HuN4 and in alleviating the oxidative damage occurring in the process. In summary, these results may provide new insights into understanding the mechanisms of HP-PRRSV infection. (C) 2012 Published by Elsevier B.V.

分类号：

  • 相关文献

[1]Contrasting silver nanoparticle toxicity and detoxification strategies in Microcystis aeruginosa and Chlorella vulgaris: New insights from proteomic and physiological analyses. Qian, Haifeng,Chen, Jun,Zhu, Kun,Fu, Zhengwei,Lu, Haiping,Lavoie, Michel,Chen, Si,Zhou, Zhongjing,Deng, Zhiping. 2016

[2]Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus. Zhang, Xin,Shi, Hong-Yan,Chen, Jian-Fei,Shi, Da,Feng, Li,Lang, Hong-Wu,Wang, Zhong-Tian. 2013

[3]Pathological and immunological characteristics of piglets infected experimentally with a HP-PRRSV TJ strain. Li, Zhenguang,He, Yanliang,Li, Shufen,Xia, Mingqi,Wu, Hua,Li, Zhenguang,Leng, Xue,Wen, Yongjun,Wang, Fengxue,Cheng, Shipeng,Wu, Hua,Xu, Xiaoqin. 2016

[4]Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs. Wang, Jinbao,Yu, Jiang,Wang, Jinbao. 2012

[5]Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation. Leng, Xue,Li, Zhenguang,Wang, Fengxue,Wang, Wei,Zhang, Xiuhua,Wu, Hua,Xia, Mingqi,Li, Xiyu,Wu, Hua.

[6]Characterizing the thymic lesions in piglets infected with attenuated strains of highly pathogenic porcine reproductive and respiratory syndrome virus. Wang, Gang,Yu, Ying,Tu, Yabin,Li, Yuming,Tong, Jie,Zhang, Chong,Liu, Yonggang,Li, Aidong,Jiang, Chenggang,Wang, Shujie,Tao, Ye,Cai, Xuehui,Zhang, Chong.

[7]Pathogenicity and Distribution of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Pigs. Hu, S. P.,Zhang, Z.,Liu, Y. G.,Tian, Z. J.,Wu, D. L.,Cai, X. H.,He, X. J.. 2013

[8]Molecular cloning and characterizations of porcine SAMHD1 and its roles in replication of highly pathogenic porcine reproductive and respiratory syndrome virus. Yang, Shen,Shan, Tongling,Zhou, Yanjun,Jiang, Yifeng,Tong, Wu,Liu, Fei,Wen, Feng,Zhang, Qingzhan,Tong, Guangzhi,Shan, Tongling,Zhou, Yanjun,Tong, Guangzhi.

[9]Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay. Yang, Yang,Qin, Xiaodong,Zhang, Zhidong,Sun, Yingjun,Chen, Ting.

[10]Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Wang, Qian,Peng, Jinmei,An, Tongqing,Tian, Zhijun,Cai, Xuehui,Li, Yanwei,Dong, Hong,Wang, Li,Dong, Hong,Wang, Li,Yang, Xufu. 2017

[11]Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. Li, Jiangnan,Wang, Shengnan,Li, Changyao,Wang, Chunlai,Liu, Yonggang,Wang, Gang,He, Xijun,Hu, Liang,Liu, Yuanyuan,Cui, Mengmeng,Bi, Caihong,Shao, Zengyu,Cai, Xuehui,Huang, Li,Weng, Changjiang,Wang, Xiaojie,Xiong, Tao.

[12]A comparison of Fe, Zn, Cu, Cr, Cd and Pb concentration in serum determined by ICP-AES using different sample preparation procedures. Zhang, XL,Cao, H,Yang, LX,Wang, LX,Duan, YY,Zhang, SK,Yang, XK. 1999

[13]The effect of implanting an antigen release device on lactoferrin concentration in serum and milk. Cheng, J. B.,Wang, J. Q.,Bu, D. P.,Liu, G. L.,Zhang, C. G.,Wei, H. Y.,Zhou, L. Y.,Wang, J. Z.,Iaschi, S. P. A.,Tay, K. G.,Zhang, C. G.,Wei, H. Y.,Zhou, L. Y.,Tay, K. G.. 2008

[14]Determination of optimal fasting time before blood sampling to get baseline data on serum biochemical characteristics in juvenile turbot (Scophthalmus maximus). Jia, Yudong,Huang, Bin,Gao, Yunhong,Chen, Xiatian. 2018

[15]Heterosis of haernolymph analytes of two geographic populations in Chinese shrimp Fenneropenaeus chinensis. Yang, Cuihua,Kong, Jie,Wang, Qingyin,Liu, Qinghui,Tian, Yi,Luo, Kun. 2007

[16]Responses of Glutathione-Related Antioxidant Defense System in Serum of Nile Tilapia (Oreochromis niloticus) Exposed to Sublethal Concentration of Methomyl and Recovery Pattern. Meng, Shun-Long,Qu, Jian-Hong,Fan, Li-Min,Qiu, Li-Ping,Chen, Jia-Zhang,Xu, Pao,Meng, Shun-Long,Chen, Jia-Zhang,Xu, Pao.

[17]Experimental infection with bovine ephemeral fever virus and analysis of its antibody response cattle. Zheng, F. Y.,Chen, Q. W.,Li, Z.,Gong, X. W.,Wang, J. D.,Yin, H..

[18]Effect of methomyl on sex steroid hormone and vitellogenin levels in serum of male tilapia (Oreochromis niloticus) and recovery pattern. Meng, Shun-Long,Qiu, Li-Ping,Hu, Geng-Dong,Fan, Li-Min,Song, Chao,Zheng, Yao,Wu, Wei,Qu, Jian-Hong,Li, Dan-Dan,Chen, Jia-Zhang,Xu, Pao.

[19]Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows. Min, Li,Shi, Bao-lu,Zheng, Nan,Wang, Jia-qi,Min, Li,Yang, Hong-jian,Cheng, Jian-bo. 2015

[20]Levels of Cu, Mn, Fe and Zn in Cow Serum and Cow Milk: Relationship with Trace Elements Contents and Chemical Composition in Milk. Wang, Hui,Liu, Yongming,Qi, Zhiming,Wang, Shengyi,Liu, Shixiang,Dong, Shuwei,Xia, Xinchao,Li, Shengkun,Liu, Zhiqi. 2014

作者其他论文 更多>>

微信小程序