Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus

文献类型： 外文期刊

第一作者： Zhang, Shu-zhen

作者： Zhang, Shu-zhen;Wu, Su-ran;Yu, Nai-tong;Wang, Jian-hua;Liu, Zhi-xin;Kulye, Mahesh;Zeng, Hong-mei

作者机构：

关键词： bacteria-expressed dsRNA;bioassay;ecdysteroid signaling;phylogenetic analysis;quantitative real-time PCR;RNA interference

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2012 年 12 卷

页码：

收录情况： SCI

摘要： RNA interference (RNAi) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. Development of reliable methods for production and delivery of double-stranded RNA (dsRNA) is the major challenge for efficient pest control. In this study, Chilo infuscatellus Snellen (Crambidae: Lepidoptera) was fed with CiHR3 dsRNA expressed in bacteria or synthesized in vitro. The dsRNA ingested by C. infuscatellus successfully triggered silencing of the molt-regulating transcription factor CiHR3, an important gene for insect growth and development, and caused significant abnormalities and weight loss in insects within seven days of treatment. This study is an ideal example of feeding-based RNAi mediated by dsRNA expressed in bacteria or synthesized in vitro. The results also suggested that feeding-based RNA interference is a potential method for the management of C. infuscatellus.

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