Multivariate analysis linkage of phyllospheric microbial community of transgenic cotton from SGK321 to Cry1Ac: A temporal expression dynamics

文献类型： 外文期刊

第一作者： Pan, Jiangang

作者： Pan, Jiangang;Cui, Mengmeng;Hu, Qing;Ma, Anzhou;Bai, Zhihui;Qi, Hongyan;Pan, Jiangang;Zhang, Hongxun;Guo, Hongguang;Pan, Jiangang;Yang, Dianlin

作者机构：

关键词： Bt cotton;microbial diversity;16S rRNA;18S rRNA;phyllosphere

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2012 年 6 卷 25 期

页码：

收录情况： SCI

摘要： The effects of transgenic plant on ecological and food biosafety have attracted considerable attention recently due to the exploding number of commercialization genetic modified plants. Here, we examined the changes of bacterial and fungal community compositions in the phyllosphere of Cry1Ac Bacillus thuringiensis (Bt) cotton SGK321 by molecular fingerprint method at four major growth stages. The phyllospheric bacteria diversity of SGK321 was decreased at budding stage only, indicated by Shannon index. A slight increase of diversity and abundance of fungal community could be detected at different sampling stage except the seeding stage. Fungal community showed a tendency to be affiliated with the expression of Cry1Ac protein which was detected by enzyme-linked immunosorbent assay (ELISA) method at both the seeding and budding stages. Results suggested that, compared to bacteria, fungi in phyllosphere of transgenic cotton SGK321 might be more susceptible to Cry1Ac protein, which includes Cochliobolus sp., Setosphaeria sp., Blakeslea trispora, Cryptococcus neoformans and Cryptococcus sp. Furthermore, the seasonal and temporal parameters during the growth stages were proved to be the most important factor relating with the shift of phyllospheric microorganism communities.

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