Novel Haplotype in the HHEX Gene Promoter Associated with Body Length in Pigs

文献类型： 外文期刊

第一作者： Luo, Yabiao

作者： Luo, Yabiao;Xu, Qiao;Xue, Mingming;Wang, Yubei;Yang, Xiaoyang;Chan, Shuheng;Tang, Qiguo;Fang, Meiying;Xu, Qiao;Wang, Feng;Sun, Ruiping;Chao, Zhe;Fang, Meiying

作者机构：

关键词： HHEX; body length; expression pattern; gene polymorphism; promoter activity

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 2 期

页码：

收录情况： SCI

摘要： The screening of important candidate genes and the identification of genetic markers are important for molecular selection in the pig industry. The hematopoietically expressed homeobox (HHEX) gene plays an important role in embryonic development and organogenesis; however, the genetic variation and expression pattern of the porcine HHEX gene remains to be clarified. In this study, semiquantitative RT-PCR and immunohistochemistry results showed the specific expression of the HHEX gene in porcine cartilage tissues. A novel haplotype consisting of two SNPs rs80901185 (T > C) and rs80934526 (A > G) was detected in the promoter region of the HHEX gene. The expression of the HHEX gene was significantly higher in Yorkshire pigs (TA haplotype) than in Wuzhishan pigs (CG haplotype), and a population analysis showed that this haplotype was significantly associated with body length. An analysis subsequently revealed that the -586 to -1 bp region of the HHEX gene promoter showed the highest activity. Furthermore, we found that the activity of the TA haplotype was significantly higher than that of the CG haplotype by changing the potential binding of transcription factors YY1 and HDAC2. In summary, we conclude that the porcine HHEX gene may contribute to the breeding of pigs for body length traits.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序