Determination of Gibberellic Acid and alpha-Naphthlene Acetic Acid in Tea by Ultra-pressure Liquid Chromatography Tandem Mass Spectrometry with Dispersive Solid Phase Extraction

文献类型： 外文期刊

第一作者： Chen Hong-Ping

作者： Chen Hong-Ping;Liu Xin;Wang Chuan-Pi;Yin Peng

作者机构：

关键词： Gibberellic acid;alpha-Naphthlene acetic acid;Dispersive solid phase extraction;Ultra-pressure liquid chromatography tandem mass spectrometry

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2012 年 40 卷 7 期

页码：

收录情况： SCI

摘要： A rapid analytical method based on ultra-pressure liquid chromatography tandem mass has been developed for the determination of plant hormones gibberellic acid (GA(3)) and alpha-naphthlene acetic acid (NAA) in tea. The analytes were extracted from the homogenizated sample using methonolic, cleaned-up with mixture of four sorbents Florisil, graphitized carbon black (GCB), primary secondary amine (PSA) and C-18. GA(3) and NAA were separated on HSS C-18 column (100 mm X 2.0 mm, 1.8 mu m) and determined on UPLC-MS/MS by negative-electrospray ionization (ESI-) using multiple reaction monitoring (MRM). Quantification was done by the external standard curves. The linear range of GA(3) and NAA was 0.05-5.0 mg/kg and 0.10-5.0 mg/kg respectively, the correlation coefficient (r) of both plant hormone standard curve was more than or equal to 0.9990, the limit of quantification of GA(3) was 0.05 mg/kg and that of NAA was 0.10 mg/kg. The recoveries of three levels spiked with 0.1, 0.5 and 1.0 mg/kg were 85.0%-86.8% for GA(3) and 82.9%-84.4% for NAA, the relative standard deviations of both plant hormones were less than or equal to 4.5%. The method is simple, accurate and sensitive for the simultaneous determination of GA(3) and NAA residual in tea.

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