文献类型: 外文期刊
第一作者: Wu, Sheng-Jun
作者: Wu, Sheng-Jun;Gao, Hui-Lan;Zhang, Yong-Hua;Li, Shi-Dong;Xie, Bing-Yan
作者机构:
关键词: Gliocladium catenulatum;chitinases;purification;characteristics
期刊名称:AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH ( 影响因子:0.539; 五年影响因子:0.564 )
ISSN: 1996-0808
年卷期: 2012 年 6 卷 20 期
页码:
收录情况: SCI
摘要: Chitinase was isolated and purified from Gliocladium catenulatum through ammonium sulfate sedimentation, sephadex G-25 gel filtration, deionization, ultra filtration condensation, negative ion exchange separation, and non-denaturing gel electrophoresis. A 51 kDa chitinase was purified from the fungus through sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum temperature and pH for chitinase activity were 60 degrees C and pH 6.0, respectively. The enzyme solution was stable from 20 to 40 degrees C and pH 4 to 5. Co2+ and Ca2+ were conducive to the enzyme activity, whereas the Fe3+, Cu2+, and Ag+ evidently inhibited the enzyme activity. Fe2+, Na+, K+, Zn2+, and Mn2+ showed less inhibitory effects on the enzyme activity. The K-m of the chitinase was 2.832 mg ml(-1). The chitanase were found to inhibit the hyphal growth, conidial germination and sclerotial germination of various plant pathogenic fungi.
分类号:
- 相关文献
作者其他论文 更多>>
-
Comprehensive Analysis of the Complete Mitochondrial Genome of Rehmannia chingii: An Autotrophic Species in the Orobanchaceae Family
作者:Han, Ying;Jin, Xin-Jie;Zhang, Yong-Hua;Feng, Yan-Lei;Feng, Yan-Lei;Wang, Jie;Wu, Zhi-Qiang;Zhu, Shan-Shan;Zhu, Shan-Shan;Jin, Xin-Jie;Zhang, Yong-Hua
关键词:mitogenome; Rehmannia chingii; Orobanchaceae; organelle genome; RNA editing
-
Transcriptomic Response of Clonostachys rosea Mycoparasitizing Rhizoctonia solani
作者:Sun, Zhan-Bin;Yu, Shu-Fan;Hu, Ya-Feng;Song, Han-Jian;Sun, Man-Hong;Li, Shi-Dong
关键词:biocontrol; comparative transcriptomic analysis; differentially expressed genes; fungal plant pathogens; mycoparasitism
-
The Adenylate Cyclase-Encoding Gene crac Is Involved in Clonostachys rosea Mycoparasitism
作者:Yu, Shu-Fan;Sun, Zhan-Bin;Hu, Ya-Feng;Ren, Qing;Xu, Jia-Liang;Song, Han-Jian;Li, Shi-Dong;Sun, Man-Hong
关键词:Clonostachys rosea; adenylate cyclase; gene knockout and complementation; differentially expressed genes
-
Combination of Bacillus and Low Fertigation Input Promoted the Growth and Productivity of Chinese Cabbage and Enriched Beneficial Rhizosphere Bacteria Lechevalieria
作者:Zhang, Shi-Chang;Zhang, Yu-Lu;Guo, Xiao-Jing;Li, Shi-Dong;Guo, Rong-Jun;Zhang, Shi-Chang;Luo, Ming
关键词:soil water potential; growth promotion; Bacillus; rhizosphere microbial community; plant hormone
-
The Co-Association of Enterobacteriaceae and Pseudomonas with Specific Resistant Cucumber against Fusarium Wilt Disease
作者:Zhang, Yu-Lu;Guo, Xiao-Jing;Huang, Xin;Guo, Rong-Jun;Lu, Xiao-Hong;Li, Shi-Dong;Huang, Xin;Zhang, Hao
关键词:cucumber Fusarium wilt (CFW); Fusarium oxysporum f; sp; cucumerinum (Foc); resistant and susceptible cultivars; root microbiota; suppressive functions
-
A Meloidogyne incognita effector Minc03329 suppresses plant immunity and promotes parasitism
作者:Zhou, Jing-Jing;Zhang, Xiao-Ping;Liu, Rui;Ling, Jian;Li, Yan;Yang, Yu-hong;Xie, Bing-yan;Zhao, Jian-long;Mao, Zhen-chuan
关键词:Meloidogyne incognita; effector; C-type lectin; pathogenicity; plant immunity
-
Complete Genome Sequence of Achromobacter sp. Strain 77, a Hyphosphere Isolate of Fusarium oxysporum f. sp. cucumerinum
作者:Li, Ji-Hong;Guo, Rong-Jun;Ma, Gui-Zhen;Li, Ji-Hong;Jing, Yu-Ling;Guo, Rong-Jun;Li, Shi-Dong
关键词: