Identification of an acidic alpha-amylase from Alicyclobacillus sp A4 and assessment of its application in the starch industry

文献类型： 外文期刊

第一作者： Bai, Yingguo

作者： Bai, Yingguo;Huang, Huoqing;Meng, Kun;Shi, Pengjun;Yang, Peilong;Luo, Huiying;Luo, Chunliang;Feng, Yukun;Yao, Bin;Zhang, Wei

作者机构：

关键词： Alicyclobacillus sp A4;Acidic alpha-amylase;Raw starch digesting enzyme

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2012 年 131 卷 4 期

页码：

收录情况： SCI

摘要： An acidic alpha-amylase was purified from thermoacidophilic Alicyclobacillus sp. A4 by ion exchange chromatography with 22% recovery, and showed a molecular mass of 64 kDa by SOS-PAGE. Its amino acid sequence was determined by sequencing three internal peptides and the complete genome of strain A4, and shared highest identity (64%) with Alicyclobacillus acidocaldarius alpha-amylase. Compared with other reported alpha-amylases, the purified enzyme had some distinct characteristics. The optimal activity was found to occur at 75 degrees C and pH 4.2, similar to the glucamylase widely used in the starch industry. The enzyme was Ca2+ independent, and had strong ability to digest raw starch (96.71%) with commercial glucamylase in one step. These properties of the purified enzyme make up the deficiency of the commercial alpha-amylases currently used and avoid repeated adjustment of pH and temperature in double-enzymatic sugar-making process. The purified enzyme will be commercially valuable in the starch industry. (C) 2011 Elsevier Ltd. All rights reserved.

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