Establishment and evaluation of a novel TaqMan probe-based real-time PCR for detection of Canine Parvovirus

文献类型： 外文期刊

第一作者： Shi, Lijun

作者： Shi, Lijun;Zhao, Zhanzhong;Wang, Jing;Yuan, WeiFeng;Zhu, Hongfei;Li, Gang;Yin, Huiqiong;Zhang, Jingang

作者机构：

关键词： Canine Parvovirus;VP2 gene;real-time polymerase chain reaction

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2012 年 6 卷 13 期

页码：

收录情况： SCI

摘要： Rapid, reliable and quantitative assays of virus universal detection are essential for fighting against Canine Parvovirus (CPV). A rapid fluorescence probe-based real-time polymerase chain reaction (RTPCR) for detecting Canine Parvovirus was established. Primers were designed according to VP2 protein gene by Primer Premier 5.0. In response, a sensitive assay with the TaqMan probe was developed and validated. Ten-fold successive dilutions of positive CPV DNA plasmids were used to measure the sensitivity of (RT-PCR). Amplifying curve showed that this method could successfully amplify 102 copies/mu L CPV gene, meanwhile reference Porcine Parvovirus (PPV), Porcine Circovirus (PCV) and blank control were all negative. The assay system showed high reproducibility with CV<3%. Thus the newly-built real-time PCR assay was indicated to be a rapid, sensitive and specific test for detecting CPV.

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