Discovery and characterization of Coturnix chinensis avian ss-defensin 10, with broad antibacterial activity
文献类型: 外文期刊
第一作者: Ma, Deying
作者: Ma, Deying;Lin, Lijuan;Zhang, Kexin;Wang, Ruiqin;Lin, Lijuan;Zhang, Kexin;Han, Zongxi;Shao, Yuhao;Wang, Ruiqin;Liu, Shengwang
作者机构:
关键词: avian ss-defensin 10;quail;expression;synthetic peptide;recombinant peptide;antimicrobial activity
期刊名称:JOURNAL OF PEPTIDE SCIENCE ( 影响因子:1.905; 五年影响因子:2.244 )
ISSN: 1075-2617
年卷期: 2012 年 18 卷 4 期
页码:
收录情况: SCI
摘要: A novel avian beta-defensin (AvBD), AvBD10, was discovered in the liver and bone marrow tissues from Chinese painted quail (Coturnix chinensis) in the present study. The complete nucleotide sequence of quail AvBD10 contains a 207-bp open reading frame that encodes 68 amino acids. The quail AvBD10 was expressed widely in all the tissues from quails except the tongue, crop, breast muscle, and thymus and was highly expressed in the bone marrow. In contrast to the expression pattern of AvBD10 in tissues from quail, the chicken AvBD10 was expressed in all 21 tissues from the layer hens investigated, with a high level of expression in the kidney, lung, liver, bone marrow, and Harderian glands. Recombinant glutathione S-transferase (GST)-tagged AvBD10s of both quail and chicken were produced and purified by expression of the two cDNAs in Escherichia coli, respectively. In addition, peptide according to the respective AvBD10s sequence was synthesized, named synthetic AvBD10s. As expected, both recombinant GST-tagged AvBD10s and synthetic AvBD10s of quail and chicken exhibited similar bactericidal properties against most bacteria, including Gram-positive and Gram-negative forms. However, no significant bactericidal activity was found for quail recombinant GST-tagged AvBD10 against Salmonella choleraesuis or for chicken recombinant GST-tagged AvBD10 against Proteus mirabilis. Copyright (c) 2012 European Peptide Society and John Wiley & Sons, Ltd.
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