Molecular cloning and characterization of a novel stress responsive gene in alfalfa

文献类型： 外文期刊

第一作者： Sun, Y.

作者： Sun, Y.;Long, R.;Yang, Q.;Kang, J.;Chao, Y.;Wu, M.;Long, R.;Wang, P.;Qin, Z.

作者机构：

关键词： Allium cepa;gene expression;Medicago sativa;PB1 domain;phylogenetic tree;RACE;subcellular localization

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN： 0006-3134

年卷期： 2012 年 56 卷 1 期

页码：

收录情况： SCI

摘要： A suppression subtraction hybridization (SSH) cDNA library of alfalfa (Medicago sativa L.) cv. Zhongmu NO. 1 had been constructed to identify differentially expressed genes under stress. Based on the sequence of a 460 bp expressed sequence tags (ESTs), a cDNA of 1652 bp was cloned by rapid amplification of cDNA ends (RACE) method. This gene (MsPBL) was predicted to encode a 434-amino-acid protein, which contained a Phox and Bem1 (PB1) domain. PB1 domain is a functional domain comprising about 80 amino acid residues, which exists in many signal transduction proteins and mediates dimerization in the proteins. PB1 domain is mostly involved in two cell signal transduction pathways: MAPK and NF-KB. When fused to the green fluorescent protein, we found MsPBL localization in the nucleus of onion (Allium cepa L.) epidermal cells. The transcripts of MsPBL rose significantly when alfalfa was treated with 300 mM NaCl, 0.1 mM ABA, and 20 % polyethylene glycol (PEG-6000). These results indicated that MsPBL may be functional within the nucleus as a signal transduction protein to allow alfalfa to rapidly respond to the environmental stress signals.

分类号：

  • 相关文献

[1]Expression of two uricase (Nodulin-35) genes in a non-ureide type legume, Medicago sativa. Cheng, XG,Nomura, M,Takane, K,Kouchi, H,Tajima, S. 2000

[2]Cloning and characterization of gene encoding a Mn-containing superoxide dismutase in Eutrema halophilum. Xu, X. J.,Zhou, Y. J.,Bu, H. H.,Feng, J. C.,Ren, D. T.,Wang, G. Y.. 2014

[3]Cloning and Expression of Calcium-Dependent Protein Kinase (CDPK) Gene Family in Common Tobacco (Nicotiana tabacum). Tai Shuai-shuai,Liu Guan-shan,Sun Yu-he,Chen Jia. 2009

[4]Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia. Wan, Yongqing,Mao, Mingzhu,Yang, Qi,Li, Guojing,Wang, Ruigang,Wan, Dongli,Yang, Feiyun,Mandlaa. 2018

[5]Isolation and Characterization of Ftsz Genes in Cassava. Geng, Meng-Ting,Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Geng, Meng-Ting,Min, Yi,Chen, Xia,Fan, Jie,Yuan, Shuai,Wang, Lei,Zhang, Fan,Shang, Lu,Wang, Yun-Lin,Hu, Xin-Wen,Sun, Chong. 2017

[6]Molecular cloning and characterization of a gene regulating flowering time from Alfalfa (Medicago sativa L.). Zhang, Tiejun,Chao, Yuehui,Kang, Junmei,Ding, Wang,Yang, Qingchuan.

[7]Isolation and functional characterization of a Medicago sativa L. gene, MsLEA3-1. Sun, Yan,Sun, Yan,Bai, Yongqin,Yang, Qingchuan,Kang, Junmei,Chao, Yuehui,Gruber, Margaret.

[8]Cloning, localization and expression analysis of two fw2.2-like genes in small- and large-fruited pear species. Tian Jia,Zeng Bin,Li Jiang,Luo Shu-ping,Li Xiu-gen,Wu Bin,Wu Bin.

[9]Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses. Wang, Lijuan,Ma, Fei,Shen, Wenbiao,Xu, Sheng,Wang, Ren,Zheng, Tianqing,Chen, Huiping,Chen, Huiping.

[10]Induction of heme oxygenase-1 with beta-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa. Fu, Guangqing,Cui, Weiti,Wang, Yanqin,Shen, Wenbiao,Zhang, Liefeng,Ren, Yong,Zheng, Tianqing. 2011

[11]Physiological and antioxidant responses of three leguminous species to saline environment during seed germination stage. Wang, Xiaoshan,Gu, Hongru,Wang, Xiaoshan,Zhao, Guoqi. 2009

[12]Effect of rhizobia symbiosis on lignin levels and forage quality in alfalfa (Medicago sativa L.). Zhang, Zhiqiang,Chang, Leqin,Cao, Yuman,Zhang, Tong,Wang, Yafang,Liu, Yushi,Zhang, Pan,Hu, Tianming,Yang, Peizhi,Zhang, Zhiqiang,Shao, Linhui,Zhang, Pan,Sun, Xiaoqin,Wu, Yajun.

[13]Methane alleviates alfalfa cadmium toxicity via decreasing cadmium accumulation and reestablishing glutathione homeostasis. Gu, Quan,Chen, Ziping,Cui, Weiti,Zhang, Yihua,Yu, Xiuli,Wang, Qingya,Shen, Wenbiao,Hu, Huali. 2018

[14]Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.). Li, Zhenyi,Long, Ruicai,Zhang, Tiejun,Wang, Zhen,Zhang, Fan,Yang, Qingchuan,Kang, Junmei,Sun, Yan.

[15]A rapid screening method for evaluating resistance of alfalfa (Medicago sativa L.) to Fusarium root rot. Cong, L. L.,Wang, Z.,Kang, J. M.,Zhang, T. J.,Yang, Q. C.,Cong, L. L.,Sun, Y.,Biligetu, B.. 2018

[16]Effects of dietary alfalfa flavonoids on the performance, meat quality and lipid oxidation of growing rabbits. Dabbou, Sihem,Schiavone, Achille,Gasco, Laura,Rotolo, Luca,Gasco, Laura,Schiavone, Achille,Gai, Francesco,Pozzo, Luisa,Tong, Jian Ming,Dong, Xiao Fang,Rubiolo, Patrizia. 2018

[17]Genetic diversity and phenotypic variation for drought resistance in alfalfa (Medicago sativa L.) germplasm collected for drought tolerance. Zhang, Tiejun,Kesoju, Sandya,Hu, Jinguo,Yu, Long-Xi,Zhang, Tiejun,Kesoju, Sandya,Greene, Stephanie L.,Fransen, Steven. 2018

[18]Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.. Li, Zhen-yi,Long, Rui-cai,Zhang, Tie-jun,Yang, Qing-chuan,Kang, Jun-mei.

[19]Expression of the alfalfa CCCH-type zinc finger protein gene MsZFN delays flowering time in transgenic Arabidopsis thaliana. Chao, Yuehui,Zhang, Tiejun,Yang, Qingchuan,Kang, Junmei,Qin, Zhihui,Sun, Yan,Gruber, Margaret Yvonne,Qin, Zhihui. 2014

[20]Genetic relationships among alfalfa gemplasms resistant to common leaf spot and selected Chinese cultivars assessed by sequence-related amplified polymorphism (SARP) markers. Yuan, Qinghua,Wang, Yu,Li, Xiang-lin,Gao, Jianming,Gui, Zhi,Wang, Shuang,Zhao, Ximan,Xia, Buxian. 2011

作者其他论文 更多>>

微信小程序