Maternal control of seed oil content in Brassica napus: the role of silique wall photosynthesis

文献类型: 外文期刊

第一作者: Hua, Wei

作者: Hua, Wei;Zhan, Gao-Miao;Liu, Jing;Li, Jun;Wang, Xin-Fa;Liu, Gui-Hua;Wang, Han-Zhong;Li, Rong-Jun

作者机构:

关键词: silique wall;maternal effects;photosynthesis;seed oil content;454 pyrosequencing;Brassica napus

期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )

ISSN: 0960-7412

年卷期: 2012 年 69 卷 3 期

页码:

收录情况: SCI

摘要: Seed oil content is an important agronomic trait in rapeseed. However, our understanding of the regulatory processes controlling oil accumulation is still limited. Using two rapeseed lines (zy036 and 51070) with contrasting oil content, we found that maternal genotype greatly affects seed oil content. Genetic and physiological evidence indicated that difference in the local and tissue-specific photosynthetic activity in the silique wall (a maternal tissue) was responsible for the different seed oil contents. This effect was mimicked by in planta manipulation of silique wall photosynthesis. Furthermore, the starch content and expression of the important lipid synthesis regulatory gene WRINKLED1 in developing seeds were linked with silique wall photosynthetic activity. 454 pyrosequencing was performed to explore the possible molecular mechanism for the difference in silique wall photosynthesis between zy036 and 51070. Interestingly, the results suggested that photosynthesis-related genes were over-represented in both total silique wall expressed genes and genes that were differentially expressed between genotypes. A potential regulatory mechanism for elevated photosynthesis in the zy036 silique wall is proposed on the basis of knowledge from Arabidopsis. Differentially expressed ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)-related genes were used for further investigations. Oil content correlated closely with BnRBCS1A expression levels and Rubisco activities in the silique wall, but not in the leaf. Taken together, our results highlight an important role of silique wall photosynthesis in the regulation of seed oil content in terms of maternal effects.

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