Detection of Cryptosporidium Species in Feces from Snakes

文献类型： 外文期刊

第一作者： Chu, Y. H.

作者： Chu, Y. H.;Chen, J. X.;Ai, L.;Cai, Y. C.;Chen, S. H.;Li, J.;He, G. S.

作者机构：

关键词： Cryptosporidium serpentis;snakes;morphology;polymerase chain reaction;prevalence;sequences

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 21 期

页码：

收录情况： SCI

摘要： Cryptosporidiosis is an important gastrointestinal disease in snakes. In the current study, 143 feces samples of cobra snakes and Oriental rat-snake were examined for the presence of cryptosporidia by morphology and Polymerase Chain Reaction (PCR) Method targeting a part of the 18S ribosomal RNA gene. A consecutive sequencing reaction was used to identify the cryptosporidian species present in PCR-positive samples. The oocyst of Cryptosporidium serpent is was found in 19 out of 143 (13.29%). The results stress the importance for diagnostic methods to be specific for Cryptosporidium species especially in snakes.

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