Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)

文献类型: 外文期刊

第一作者: Pang, C. Y.

作者: Pang, C. Y.;Deng, T. X.;Yang, C. Y.;Yang, B. Z.;Liang, X. W.;Tang, D. S.;Jiang, H.

作者机构:

关键词: Molecular cloning;GC-rich;rDNA;rRNA;Bubalus bubalis

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2012 年 11 卷 3 期

页码:

收录情况: SCI

摘要: The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, including three rRNA genes and two internal transcribed spacers (ITS), which we named 18S rRNA, ITS1, 5.8S rRNA, ITS2 and 28S rRNA. We tested and optimized conditions for cloning these complicated rDNA sequences, including specific rules of primer design, improvements in the reaction system, and selection of the DNA polymerase.

分类号:

  • 相关文献

[1]Strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the China/Indochina border region. Zhang, Yi,Lu, Yongfang,Li, Qiang,Wang, Yachun,Zhang, Yuan,Yindee, Marnoch,Li, Kuan-Yi,Ju, Yu-Ten,Kuo, Hsiao-Yun,Ye, Shaohui,Faruque, Md Omar,Vu Chi Cuong,Lan Doan Pham,Bouahom, Bounthong,Yang, Bingzhuang,Liang, Xianwei,Cai, Zhihua,Vankan, Dianne,Manatchaiworakul, Wallaya,Kowlim, Nonglid,Wajjwalku, Worawidh,Duangchantrasiri, Somphot,Colenbrander, Ben,Lenstra, Johannes A.,Beerli, Peter,Barker, J. Stuart F..

[2]Efficient isolation of high quality RNA from tropical palms for RNA-seq analysis. Xiao, Yong,Yang, Yaodong,Cao, Hongxing,Fan, Haikuo,Lei, Xintao,Ma, Zilong,Xiao, Yong,Yang, Yaodong,Cao, Hongxing,Fan, Haikuo,Lei, Xintao,Mason, Annaliese S.,Mason, Annaliese S.,Xia, Zhihui,Huang, Xi. 2012

[3]Intraspecific polymorphism of rDNA among five Nosema bombycis isolates from different geographic regions in China. Liu, Handeng,Pan, Guoqing,Luo, Bo,Li, Tian,Zhou, Zeyang,Liu, Handeng,Zhou, Zeyang,Yang, Qiong,Vossbrinck, Charles R.,Debrunner-Vossbrinck, Bettina A.. 2013

[4]Structural characterization and applications of ITS2 from rice leaffolders Cnaphalocrocis medinalis and Marasmia patnalis (Lepidoptera: Pyralidae). Yang, Yajun,Wu, Zhihong,Xu, Hongxing,Zheng, Xusong,Lu, Zhongxian. 2017

[5]Diversification of the P genome among Agropyron Gaertn. (Poaceae) species detected by FISH. Zhao, Yan,Wang, Junjie,Zhang, Zhong,Zhao, Yan,Wang, Junjie,Zhang, Zhong,Zhao, Yan,Wang, Junjie,Zhang, Zhong,Xie, Jihong,Dou, Quanwen. 2017

[6]Genetic polymorphism of ferula mushroom growing on Ferula sinkiangensis. Zhang, JX,Huang, CY,Ng, TB,Wang, HX.

[7]A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Peng, DL,Moens, M.

[8]Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH. Yimei Gan,Fang Liu,Renhai Peng,Chunying Wang,Shaohui Li,Xiangdi Zhang,Yuhong Wang,Kunbo Wang. 2012

[9]Bipolaris eleusines, a potential mycoherbicide candidate for control of barnyardgrass ( Echinochloa crus-galli). Zhang, Jianping,Duan, Guifang,Zhou, Yongjun,Yang, Shuang,Yu, Liuqing,Peng, Gary. 2014

[10]Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns. Li, Kun-Peng,Wu, Yun-Xiang,Wang, Yan,Lu, Xing-Ming,Li, Zong-Yun,Han, Yong-Hua,Zhao, Hong,Xu, Yong,Wang, Ji-Ming. 2016

[11]Expression, characterization, and antimicrobial ability of T4 lysozyme from methylotrophic yeast Hansenula polymorpha A16. Wang Nan,Li GangQiang,Sun Ning,Liu DeHu,Wang YueJu. 2011

[12]Divergence between C-melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern. Li, Kunpeng,Sun, Jianying,Li, Zongyun,Han, Yonghua,Wang, Huaisong,Wang, Jiming.

[13]A new entomopathogenic nematode, Steinernema hebeiense sp n. (Rhabditida : Steinernematidae), from North China. Chen, Shulong,Li, Xiuhua,Yan, Aihua,Spiridonov, Sergei E.,Moens, Maurice. 2006

[14]Cytogenetic Identification of a New Hexaploid Coix aquatica Cyto-type. Li, DY,Li, YC,Xue, YG,Hu, ZL,Song, YC.

[15]Karyotype characterization of Epimedium species (Berberidaceae) by fluorescent banding and physical mapping of 45S and 5S rDNA. Chen Qingfu,Ying, Diao,Wang, Youwei,Hu Zhongli,Ying, Diao,Lin Xianming,Liao Chaolin.

[16]Molecular identification of seven species of the genus Stigmaeopsis (Acari: Tetranychidae) and preliminary attempts to establish their phylogenetic relationship. Sakamoto, Hironori,Matsuda, Tomoko,Suzuki, Reiko,Gotoh, Tetsuo,Saito, Yutaka,Lin, Jian-Zhen,Zhang, Yan-Xuan,Sato, Yukie.

[17]Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.. Li, Tian,Bi, Yu-Ping,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian. 2013

[18]Molecular cloning and expression of grass carp MyoD in yeast Pichia pastoris. Wang, Lixin,Bai, Junjie,Luo, Jianren,Chen, Hong,Ye, Xing,Jian, Qing,Lao, Haihua. 2007

[19]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[20]Molecular cloning and gene expression differences of the anthocyanin biosynthesis-related genes in the red/green skin color mutant of pear (Pyrus communis L.). Yang, Ya-nan,Zhao, Guang,Zhang, Shao-ling,Gu, Chao,Wu, Jun,Yue, Wen-quan. 2013

作者其他论文 更多>>