Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows

文献类型: 外文期刊

第一作者: Liu, Li

作者: Liu, Li;Yuan, Zhengrong;Chen, Cui;Zhang, Limin;Chen, Xiaojie;Liu, Xidong;Gao, Xue;Li, Junya;Gao, Huijiang;Zhang, Lupei;Xu, Shangzhong

作者机构:

关键词: Dairy cow;mastitis;S. aureus;gene expression;PBMCs;China

期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )

ISSN: 1680-5593

年卷期: 2012 年 11 卷 14 期

页码:

收录情况: SCI

摘要: To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of Staphylococcus aureus (S. aureus) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX3C Chemokine Receptor 1 (CX(3)CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with S. aureus relative to uninfected controls (n = 31 were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of IL-8, CD14 and CCR2 gene were exhibited significantly (p<0.05) up-regulated within 2 dpi while there was no significant difference in the expression of M-CSFR, 7LR2 and CX(3)CR1 genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs.

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